真武汤对慢性心衰大鼠CaN/NFAT3信号转导通路的影响

The Effect of Zhenwu Decoction on CaN/NFAT3 Signal Pathway of Rats with Chronic Heart Failure

目的:探讨真武汤对慢性心衰大鼠钙调神经磷酸酶(CaN)/T细胞活化核因子(NFAT3)信号转导通路的影响。方法:将50只SD大鼠随机分为正常组、模型组及真武汤高、中、低剂量组,皮下注射异丙肾上腺素诱导大鼠慢性心衰。连续给药4周后,观察模型组和真武汤处理组大鼠的心肌组织病理结构变化,检测模型组和真武汤处理组大鼠的心脏质量指数、血浆BNP含量、心肌组织CaN活性和NAFT3蛋白表达情况及各组大鼠的血流动力学指标。结果:真武汤高剂量组左心室射血分数(LVEF)高于模型组(P<0.05);与模型组相比,真武汤高剂量组心脏质量指数、血浆B型利钠肽(BNP)含量、心肌组织CaN浓度、NAFT3蛋白表达水平均低于模型组(P<0.01)。结论:真武汤能改善慢性心衰大鼠心肌组织损伤,抑制心肌细胞肥大,可能与调节CaN/NFAT3信号转导通路有关。

Objective:To explore the effect of Zhenwu Decoction on the CaN/NFAT3 signaling pathway in rats with chronic heart failure.Methods:50 SD rats were randomly divided into normal group,model group,and high,medium,and low dose groups of Zhenwu Decoction.Chronic heart failure was induced by subcutaneous injection of isoproterenol.After continuous administration for 4 weeks,pathological changes were observed in the myocardial tissue of rats between the model group and the treatment groups of Zhenwu Decoction.The heart mass index,plasma BNP content,the activity of CaN and hemodynamic indicators of the rats were detected in the model group and the treatment groups of Zhenwu Decoction.Results:The left ventricular ejection fraction(LVEF) in the high-dose group of Zhenwu decoction was higher than that in the model group(P < 0.05).Compared to the model group of rats,the cardio-body ratio index,plasma B-type natriuretic peptide(BNP) content,CaN concentration in the myocardial tissue,and the expression level of NAFT3 protein were lower in the high dose of Zhenwu decoction group of rats(P < 0.01).Conclusion:Zhenwu Decoction can improve myocardial tissue damage and inhibit myocardial cell hypertrophy in rats with chronic heart failure,which may be related to regulating the CaN/NFAT3 signaling pathway.