植物纤维对MS培养液中烟酸和维生素B_(6)的水平浸润传质效应

Horizontal Infiltration and Mass Transfer Effects of Plant Fibers on Nicotinic Acid and Vitamin B_(6)in MS Culture Medium

为筛选用于所发明帘状锚固培养生物反应器培养床的纤维,模拟反应器通过纤维丝将培养液浸润传质到愈伤组织细胞的流程创制了“纤维水平浸润传质模拟装置”,结合改进的烟酸、维生素B6、维生素B1 HPLC同步检测法,流动相洗脱体系采用庚烷磺酸钠溶液∶乙腈=90∶10(V∶V)等度洗脱等,研究16种纤维集束对MS培养液中烟酸、维生素B6和维生素B1的水平浸润传质效应。结果表明,16种纤维集束水平浸润传质具有升降MS培养液中烟酸、维生素B6浓度的效应,但效应持续时间均不超过84 min,且具有较明显种类间差异,秸秆和木材纤维的作用效应弱于茎皮和竹材纤维。慈竹、青皮竹竹材纤维集束中烟酸浓度的升降较明显,在0.02~2.58μg/mL,苎麻茎皮纤维集束中烟酸浓度的升幅最高,达3.17μg/mL,是原浓度的5倍多,持续时间72 min。青皮竹、毛竹竹材纤维集束传质液中维生素B6的浓度出现下降至零点的效应,苎麻茎皮纤维集束中出现较原浓度升高2倍的高点,其他种类纤维集束中3种维生素的浓度升降较小。虽然植物纤维集束会导致传质液中3种维生素的浓度升高或降低,但作用效应的持续时间均只在纤维集束饱和吸液后的84 min内。

In order to screen the fibers used in Curtain⁃liking Anchoring Culture Bioreactor,a“Fiber Horizontal Infiltration Mass Transfer Sim⁃ulation Device”was created by simulating the process of the reactor to infiltrate and transfer the culture solution to the callus cells through the fiber silk.In combination with the improved simultaneous detection methods for nicotinic acid,vitamin B6,and vitamin B1 HPLC,the mobile phase elution system used sodium heptanesulfonate solution:acetonitrile=90∶10(V∶V)equal degree elution,etc.The effects of mass transfered by horizontal infiltration in 16 plant fiber bundles on nicotinic acid,vitamin B6 and vitamin B1 of MS medium were studied.The results showed that the horizontal infiltration mass transfer in 16 plant fiber bundles had the effect of increasing or decreasing the concentration of nicotinic acid and vitamin B6 of MS culture medium,but the effect duration was not more than 84 min,and there was a significant difference between species.The effect of herb stalk and wood fiber was weaker than that of stem bark and bamboo wood fiber.The concentration of niacin in bamboo fiber bundles of Bambusa emeiensis and Bambusa textilis showed significant changes,ranging from 0.02 to 2.58μg/mL.The highest increase for niacin concentration was observed in Boehmeria nivea stem bark fiber bundles,reaching 3.17μg/mL,which was more than five times the origi⁃nal concentration and lasted for 72 minutes.The vitamin B6 concentration in the mass transfer fluid of Bambusa textilis and Phyllostachys hetero⁃cycla fiber bundle decreased to zero,and the concentration of vitamins B6 in the mass transfer fluid through Boehmeria nivea stem bark fiber bundle increased to two times as much as the original concentration,while the concentration of three vitamins in other fiber bundles increased or decreased slightly.Although plant fiber bundle can lead to an increase or decrease of the three vitamins concentration of the mass transfer flu⁃id,the duration of the effect is only within 84 minutes after the fiber bundle were infiltrated and fulled adequately by the medium fluid.