纳米流式检测技术在细胞外囊泡临床诊疗研究中的应用

Application of Nano-Flow Cytometry in Clinical Diagnosis and Therapeutics Based on Extracellular Vesicles

细胞外囊泡(EV)是细胞分泌的极具异质性的纳米小囊泡,携带着来源于母细胞的各种生物活性分子,广泛分布于各种体液中,近年来在液体活检和疾病治疗中显示出巨大的应用前景。然而,传统流式细胞仪难以检测粒径小于300 nm的EV。基于瑞利散射和鞘流单分子荧光检测技术研发成功的纳米流式检测技术(nFCM)将EV的检测下限推进至40 nm,通过单粒子水平的多参数同时检测,可实现粒径、颗粒浓度和多种生化性状的同时分析,并可应用于EV临床诊疗研究中。

Extracellular vesicles(EV)are highly heterogeneous nanoscale vesicles secreted by cells.They carry various bioactive molecules derived from the parent cells.EV are widely distributed in various body fluids,showing enormous potential in liquid biopsy and disease treatment.However,conventional flow cytometers face challenges in detecting single EV with a diameter smaller than 300 nm.The nano-flow cytometry(nFCM)developed based on Raleigh scattering and sheath-flow single-molecule fluorescence detection has successfully pushed the detection limit of EV to 40 nm.Through multi-parameter detection at the single-particle level,nFCM enables simultaneous analysis of particle size,particle concentration,and multiple biochemical properties of individual EV.nFCM can be applied to clinical diagnosis and therapeutics based on EV.