MIAC和AQP2与宫颈癌症临床病理特征的关系

Relationship between micropeptide MIAC and AQP2 interaction and cervical cancer tumor progression

目的探讨微肽抑制肌动蛋白细胞骨架(MIAC)和水通道蛋白2(AQP2)与宫颈癌症临床病理特征的关系。方法选取2021年1月至2022年1月在上海市第一妇婴保健院妇科收治的100例宫颈癌患者为研究对象。所有患者接受根治性子宫颈切除术,收集肿瘤组织及肿瘤周围组织用于蛋白和免疫组化分析。根据肿瘤分期分析MIAC和AQP2表达,绘制Kaplan-Meier生存曲线,比较不同MIAC和AQP2表达组间的中位生存时间。通过免疫组化分析癌旁组织和宫颈癌组织中MIAC和AQP2的表达。通过免疫共沉淀实验分析MIAC与AQP2的相互作用。结果宫颈癌组织中MIAC与AQP2的蛋白表达较癌旁组织降低(MIAC:1.02±0.02 vs 1.85±0.16;AQP2:1.09±0.04 vs 1.75±0.14,P<0.05)。染色分析显示宫颈癌组织中MIAC与AQP2的蛋白表达较癌旁组织降低(MIAC:2.49±0.67 vs 6.33±1.26;AQP2:3.26±0.49 vs 7.49±1.15,P<0.05)。Ⅲ~Ⅳ病期组患者MIAC和AQP2蛋白表达较Ⅰ~Ⅱ病期组降低(P<0.05),随着宫颈癌病期的进展,MIAC和AQP2的表达水平降低差异有统计学意义(P<0.05)。Kaplan-Meier生存分析显示高MIAC表达的患者群体在整个时间范围内的生存率普遍高于低MIAC表达的患者(P<0.05),高MIAC表达与更好的生存预后相关,而低MIAC表达的患者生存率较低。与非特异性的IgG对照组相比,使用针对MIAC和AQP2的特异性抗体时,AQP2和MIAC蛋白表达升高(P<0.05),表明MIAC与AQP2之间存在相互作用(MIAC IP为1.75±0.16;AQP2 IP为1.86±0.15;IgG对照为1.04±0.02)。结论宫颈癌中微肽MIAC与AQP2的相互作用对肿瘤的发展具有重要影响。这两种蛋白的表达水平显著降低与病情的恶化相关,而高MIAC表达与更佳的生存预后相关,揭示了它们在宫颈癌进展中的关键作用。

Objective To explore the relationship between the interaction between micropeptide MIAC and AQP2 and the tumor progression of cervical cancer.Methods 100 cervical cancer patients admitted to the Department of Gynecology of Shanghai First Maternal and Infant Health Hospital from January 2021 to January 2022 were recruited as research subjects.All patients underwent radical trachelectomy,and tumor tissue and surrounding tissue were collected for protein and immunohistochemical analysis.Collect general patient information.The expression of MIAC and AQP2 was analyzed according to the tumor stage,and the Kaplan-Meier survival curve was drawn to compare the survival rates between different MIAC and AQP2 expression groups.The expression of MIAC and AQP2 in paracancerous tissues and cervical cancer tissues was analyzed by immunohistochemistry.The interaction between MIAC and AQP2 was analyzed by co-immunoprecipitation experiments.Results The expression of MIAC and AQP2 protein in cervical cancer tissues was lower than that in adjacent tissues(MIAC:1.02±0.02 vs 1.85±0.16;AQP2:1.09±0.04 vs 1.75±0.14,P<0.05).Staining analysis showed that the expression of MIAC and AQP2 protein in cervical cancer tissues was lower than that in adjacent tissues(MIAC:2.49±0.67 vs 6.33±1.26;AQP2:3.26±0.49 vs 7.49±1.15,P<0.05).The protein expressions of MIAC and AQP2 inⅢ~Ⅳpatients were lower than those inⅠ~Ⅱpatients(P<0.05),and the expression levels of MIAC and AQP2 were statistically significantly decreased with the progression of cervical cancer(P<0.05).Kaplan-Meier survival analysis showed that patients with high MIAC expression generally had higher survival rates than patients with low MIAC expression over the whole time range(P<0.05).High MIAC expression was associated with better survival outcomes,while patients with low MIAC expression had lower survival rates.Compared with non-specific IgG controls,specific antibodies against MIAC and AQP2 increased the expression of AQP2 and AQP2 proteins(P<0.05),indicating an interaction between MIAC and AQP2(MIAC IP:1.75±0.16;AQP2 IP:1.86±0.15;IgG:1.04±0.02).Conclusion The interaction between MIAC and AQP2 in cervical cancer has an important impact on tumor development.Significantly reduced expression levels of these two proteins were associated with disease progression,while high MIAC expression was associated with better survival prognosis,revealing their critical role in cervical cancer progression.