循环外泌体miR-485-3p和STYX表达与早发冠心病发病的相关性研究

Study on correlation between circulating exosome miR-485-3p and STYX expression with onset of premature coronary heart disease

目的研究循环外泌体miR-485-3p和丝氨酸/苏氨酸/酪氨酸结合蛋白(STYX)表达与早发冠心病发病风险的相关性。方法选取2023年8-12月于新乡医学院附属濮阳市人民医院住院并经冠状动脉造影或CT血管造影(CTA)确诊的早发冠心病患者50例作为研究组,另外选取同期经检查排除冠心病诊断的患者50例作为对照组,收集两组患者的一般临床资料,检测血浆外泌体miR-485-3p、STYX水平。采用Gensini评分评估研究组患者的冠状动脉病变程度。采用Spearman相关分析研究血浆外泌体miR-485-3p、STYX与低密度脂蛋白(LDL)、Gensini评分的关系。采用受试者工作特征(ROC)曲线分析血浆外泌体miR-485-3p、STYX对早发冠心病的诊断价值。采用多因素logistic回归确定早发冠心病的独立危险因素。结果与对照组比较,研究组冠心病家族史、吸烟史、LDL、血浆外泌体miR-485-3p水平均升高,血浆STYX水平降低,差异有统计学意义(P<0.05);Spearman相关分析显示,miR-485-3p与LDL(r=0.546)、Gensini评分(r=0.485)呈正相关,与STYX(r=-0.576)呈负相关(P<0.05);STYX与LDL(r=-0.389)、Gensini评分(r=-0.531)呈负相关(P<0.05)。ROC曲线显示,miR-485-3p、STYX及两者联合诊断早发冠心病的曲线下面积分别为0.821(95%CI:0.736~0.906)、0.850(95%CI:0.772~0.927)、0.899(95%CI:0.837~0.960)。结论在早发冠心病中,循环外泌体miR-485-3p表达上调,STYX表达下调,两者与冠状动脉病变程度密切相关,可作为诊断早发冠心病的潜在生物学标志物。

Objective To investigate the correlation between the expression of circulating exosome miR-485-3p and STYX with the risk of premature coronary heart disease.Methods A total of 50 inpatients with early onset coronary heart disease diagnosed by coronary angiography or CT angiography(CTA)in Affiliated Puyang Municipal People’s Hospital of Xinxiang Medical College from August to December 2023 were selected as the study group and 50 patients with excluded coronary artery disease by examination during the same period were included in the control group.The general clinical data of the two groups were collected,the plasma exosome miR-485-3p and STYX levels were detected.The degree of coronary arterial lesions in the patients of the study group was evaluated by the Gensini score.The Spearman correlation analysis was used to analyze the relationship between plasma exosome miR-485-3p and STYX with LDL and Gensini score.The receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of plasma exosome miR-485-3p and STYX in the diagnosis of premature coronary heart disease.The multivariate logistic regression was used to determine the independent risk factors for premature coronary heart disease.Results Compared with the control group,the family history of coronary heart disease,smoking history,LDL and plasma exosome miR-485-3p level in the study group were increased,the plasma STYX level was decreased and the differences were statistically significant(P<0.05);the Spearman correlation analysis showed that miR-485-3p was positively correlated with LDL(r=0.546)and Gensini score(r=0.485),and negatively correlated with STYX(r=-0.576).STYX was negatively correlated with LDL(r=-0.389)and Gensini score(r=-0.531).The ROC curve showed that the area under the curve of miR-485-3p,STYX and their combination in the diagnosis of premature coronary heart disease was 0.821(95%CI:0.736-0.906),0.850(95%CI:0.772-0.927)and 0.899(95%CI:0.837-0.960)respectively.Conclusion The expression of circulating exosome miR-485-3p in premature coronary heart disease is up-regulated and the expression of STYX is down-regulated,the both are closely related to the degree of coronary artery lesion,which could be used as the potential biomarkers for the diagnosis of premature coronary heart disease.