绣球花铝转运蛋白HmALMT11的生物信息学及其表达特性分析

Bioinformatics Analysis and Expression Profiling of the Aluminum Transporter HmALMT11 in Hydrangea macrophylla

铝激活苹果酸转运蛋白(ALMT)是植物中广泛存在的一个基因家族,其编码的蛋白质在调控植物根部酸分泌和对铝离子的响应方面起着重要作用。为了明晰大花绣球无尽夏HmALMT11的序列特征及其在逆境胁迫后的表达特征,进一步探索大花绣球无尽夏的生物学功能,为后续HmALMT11功能鉴定提供理论依据。以大花绣球无尽夏为材料,克隆得到HmALMT11基因,并对其进行生物信息学、亚细胞定位和铝胁迫响应分析。结果表明,HmALMT11包含1个1590 bp的开放阅读框,编码529个氨基酸,蛋白分子质量为59.1 ku,理论等电点为9.10。为不稳定的碱性蛋白;HmALMT11蛋白含有6个跨膜结构域,定位于细胞质膜;实时荧光定量PCR分析发现,低浓度100μmol/L和高浓度800μmol/L的Al 2(SO 4)3处理都可诱导HmALMT11在无尽夏根、茎、叶中的上调表达,且在根中相对表达量最高,具有明显的组织表达特异性。研究表明,HmALMT11蛋白可能在绣球花适应铝胁迫过程中发挥重要调控作用。

Aluminum-activated malate transporter(ALMT)gene family,as a widely distributed gene family in plants,the encoded protein plays an important role in regulating plant root acid secretion and response to aluminum ions.In order to clarify the sequence characteristics and expression features of H.macrophylla Endless Summer(HmALMT11)under abiotic stress,further exploring the biological functions of H.macrophylla Endless Summer,and providing a theoretical basis for the subsequent identification of the function of HmALMT11.HmALMT11 gene was cloned from the leaves of Hydrangea macrophylla Endless Summer,and the bioinformatics,subcellular localization and aluminum stress response analysis of this gene were also carried out.HmALMT11 contained an open reading frame of 1590 bp and encoded 529 amino acids,with a molecular weight of 59.1 ku and a theoretical isoelectric point of 9.10 was an unstable basic protein containing 6 transmembrane domains.The subcellular localization analysis revealed that the protein encoded by HmALMT11 was specifically targeted to the plasma membrane.The qRT-PCR analysis revealed that treatments with both low(100μmol/L)and high(800μmol/L)concentrations of Al 2(SO 4)3 increased the expression level of HmALMT11 in the roots,stems,and leaves of Hydrangea macrophylla Endless Summer.Notably,the highest expression level was observed in the roots,demonstrating a clear tissue-specific expression pattern.These results suggested that HmALMT11 protein might play an important role during the regulation of aluminum tolerance in hydrangea.