神经嵴研究手段的进展

Progress in research methods of studying the neural crest

神经嵴细胞是脊椎动物胚胎发育过程出现的一个广泛迁移的多能干细胞群体,谱系追踪是有关其研究的重点。150多年来,神经嵴相关研究经历了从形态学观察到单细胞水平的转变。本文对同位素标记、嵌合体、活体染料、体外克隆分析、基因条件重组、单细胞测序等研究手段在神经嵴研究中的运用进行综述。

The neural crest is a migratory,multipotent stem cell population during vertebrate embryogenesis,and lineage tracing is the focus of its research.Over the 150 years since its first discovery,understanding of the neural crest has gone through a transition from morphological observations to single-cell level.In this paper,important approaches applied to study the neural crest were reviewed including radioisotope labeling,chimeras,vital dye labeling,in vitro clonal analysis,conditional genetic recombination and single-cell sequencing.