玉米秸秆降解复配菌的构建及其协同效果评价

Construction and Evaluation Synergistic Effect of Corn Straw Degradation Microbial Compound

针对秸秆木质纤维素组成结构复杂性,充分利用不同菌株间的协同效应提升秸秆降解效率。以功能单菌株Achromobacter deleyi strain LMG 3458(A3)、Pseudomonas plecoglossicida strain NBRC(A4)、Aspergillus terreus(A)、Phanerochaete Chrysosporium(P)为试材,通过相互组配构建菌株丰度为1~4的多个复配菌,采用相关性分析和方差分析等方法探明菌株丰度对秸秆降解效率及其生物量的影响。结果表明,秸秆及木质纤维素降解率和酶活性均与复配菌组成丰度呈显著正相关关系,丰度为4时(A3+A4+A+P),其玉米秸秆、纤维素、半纤维素和木质素降解率分别为37.01%、36.27%、33.50%和31.27%,滤纸酶活性、木聚糖酶活性和漆酶活性分别为15.47、5.84和58.05 U/L,表明菌株间以及酶之间存在多样性效应。复配菌组成菌株间具有协同效应,促进秸秆高效降解,具有良好的应用前景。

In view of the complexity of lignocellulosic composition of straw,the synergistic effect between different strains was fully utilized to improve the degradation efficiency of straw.The functional strains Achromobacter deleyi strain LMG 3458(A3),Pseudomonas plecoglossicida strain NBRC(A4),Aspergillus terreus(A),Phanerochaete Chrysosporium(P)were used as test materials to obtain multiple compound microbial communities with a strain richness of 1-4,and via correlation analysis and variance analysis to explore the effect of strain richness on straw degradation efficiency and biomass.The corn straw and lignocellulose degradation ratio and enzyme activity were positively correlated with the composition abundance of the compound strains.The corn straw,cellulose,hemicellulose and lignin degradation ratio of compound microbial consortia(A3+A4+A+P)were 37.01%,36.27%,33.50%,and 31.27%,respectively,and the activities of filter paper enzyme,xylanase and laccase were 15.47,5.84 U/mL,and 58.05 U/L,respectively.The results showed that there were diversity effects among strains and enzymes.There was synergistic effect among the strains composed of the microbial consortia,which promotes the efficient degradation of straw.Therefore,these findings anticipate promising application prospects for the efficient degradation of straw through microbial consortia.