粗叶榕茎枯病病原菌的分离与鉴定

Isolation and identification of theepathogen causing stem blight of Ficus hirta

粗叶榕(Ficus hirta Vahl),又名五指毛桃、五爪龙、五指牛奶、亚桠木(海南)、南芪、土黄芪等^([1]),主要产于云南、贵州、海南、广西、广东、福建等地,富含蛋白质、多糖及黄酮类等物质,以根部入药,具有益气、健脾、润肺、祛湿等功效^([2]),是华南地区著名的药食同源植物和药材,享有“广东人参”的美誉,深受人们喜爱。

Ficus hirta(vahl)is a valuable medicinal plant as well as edible plants in southern China.In 2022,a new stem blight disease was observed to infect Ficus hirta(vahl)in Danzhou of Hainan province.The incidence of disease reached 47.5%in fields.In order to clarify the pathogen of Ficus hirta stem blight disease,ten isolates with the same morphology were isolated from blight tissue by using a conventional tissue separation method.MA2,a representative strain with excellent growth,was selected for pathogenicity test on Ficus hirta plants,observation of morphological characteristics,sequence analysis on ITS,TUB and TEF,and phylogenetic tree construction.The results showed that morphological characteristics of the isolate MA2 was similar to that of Lasiodiplodia pseudotheobromae.The isolate MA2 was clustered into one clade in phylogenetic tree with L.pseudotheobromae.The above results indicated that the stem blight of F.hirta was caused by L.pseudotheobromae.This is the first report of a stem blight disease on F.hirta in China was identified as L.pseudotheobromae.