小鼠“次髎”穴的取穴方法研究

Study on acupoint positioning method of"Ciliao(BL32)"in mice

目的:探寻小鼠“次髎”穴的取穴方法,为小鼠次髎穴的取穴提供一种准确可行的方案。方法:①取健康成年C57BL小鼠,通过解剖确定次髎穴坐标点;②取成年C57BL小鼠,随机分为实验组和对照组,各组分别采用坐标定位法和“L6棘突”为骨性标志的穴位定位方法进行取穴,比较两种取穴方法的准确率;③取成年C57BL小鼠,复制间质性膀胱炎(IC)小鼠模型,使用坐标定位法的方法进行取穴治疗,并验证其功能作用。结果:健康成年小鼠的次髎穴坐标为(±1 mm,21 mm,-5 mm),坐标定位法取穴准确率高于骨性标志定位法,且使用坐标定位法取穴电针治疗可以改善IC小鼠相关症状。结论:坐标定位法取穴较骨性标志定位法准确率更高,且具有次髎穴的功能。

Objective:To investigate the positioning method of"Ciliao(BL32)"acupoint in mice,and provide a precise and feasible approach for Ciliao(BL32)acupoint location in mice.Methods:①Healthy adult C57BL mice were taken,and the coordinate point of Ciliao(BL32)was determined by dissection.②Adult C57BL mice were selected and randomly divided into the experimental group and control group,and the coordinate positioning method and the acupoint positioning method with"L6 spinous process"as the bony sign were respectively applied for acupiont selection.The accuracy of the two acupoint selection methods were compared.③Adult C57BL mice were selected and the interstitial cystitis(IC)model of mouse was copied.The coordinate positioning method was applied for treatment and its functional effect was verified.Results:The coordinates of Ciliao(BL32)acupoint of healthy adult mice were(±1 mm,21 mm,-5 mm),and the accuracy of the coordinate positioning method was higher than that of the bone sign positioning method.Meanwhile the electroacupuncture treatment applying the coordinate positioning method could improve the related symptoms of IC mice.Conclusion:The accuracy of the coordinate positioning method is higher than that of the bone sign positioning method,and has the function of Ciliao(BL32)acupoint.