大口黑鲈肠道结构及分区特性

Intestinal Structure and Zoing Characteristics of Largemouth Bass

为了解大口黑鲈肠道的形态结构及分区特点,采用解剖学、石蜡切片—显微技术,研究大口黑鲈肠道形态及组织学结构特征。大口黑鲈肠道呈“S”形盘曲,比肠长为0.76±0.06。HE染色显示肠道组织由黏膜层、黏膜下层、肌层及外膜构成。肠管直径、肠绒毛高度和数量由前到后渐减,前肠前段管径、绒毛高度最长,平均管径和高度分别为(5 043.60±108.69)μm和(1 479.86±45.84)μm;后肠末端管径、绒毛高度最短,分别为(2 877.10±57.21)μm和(790.28±62.57)μm。肌层厚度前、后肠比中肠厚,前、后肠肌层最厚值分别为(339.76±22.56)μm和(251.59±26.94)μm,中肠肌层最厚值为(199.91±25.16)μm(P<0.05)。前肠末端的组织学结构特征,如肌层厚度、绒毛高度等与中肠结构特征相似。AB-PAS染色结果显示,大口黑鲈后肠杯状细胞密度显著高于前肠杯状细胞的密度(P<0.05)。该研究丰富了对大口黑鲈的肠道生理学认识,并为其肠道分区提供参考。

To understand the intestinal structure and zoning characteristics of largemouth bass,the morphological and histological characteristics of intestine were studied using anatomical and paraffin sectioning microscopy techniques.The intestine of largemouth bass was“S”-shaped,and the ratio of intestine length to body length was 0.76±0.06.HE-staining showed the intestinal tissue was composed of mucosal layer,submucosa,muscular layer and adventitia.The diameter of intestinal canal,the height and number of intestinal villi gradually decreased from front to back.The diameter and villus height of the anterior segment of the intestine were the longest,with an average diameter and height of(5043.60±108.69)μm and(1479.86±45.84)μm,respectively;The diameter and villus height of the distal end of the hindgut were the shortest,with values of(2877.10±57.21)μm and(790.28±62.57)μm,respectively.The thickness of the muscular layer in the anterior and posterior intestines were thicker than that in the midgut.The thickest values of the muscular layer in the anterior and posterior intestines are(339.76±22.56)μm and(251.59±26.94)μm,respectively.The thickest value of the muscular layer in the midgut is(199.91±25.16)μm(P<0.05).AB-PAS staining showed the density of goblet cells in hindgut was significantly higher than foregut(P<0.05).This study enriches the intestinal physiology and intestinal zoning of largemouth bass.