低共熔溶剂对玉米芯中多酚提取工艺的优化

Optimization of extraction process of polyphenols from corn cob with deep eutectic solvents

试验旨在优化玉米芯中多酚的提取工艺。通过筛选试验确定用于萃取的天然低共熔溶剂种类,基于单因素试验结果,以天然低共熔溶剂的含水量、液固比、超声功率和超声温度为变量,玉米芯多酚提取量为响应值,采用响应面法优化提取工艺,对比天然低共熔溶剂的多酚提取物和70%乙醇的多酚提取物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基的清除能力。结果显示,在天然低共熔溶剂含水量31%、液固比28 mL/g、超声功率350 W、超声温度61℃条件下,玉米芯多酚的提取量最高,可达17.49 mg/g,且提取产物的抗氧化性强于70%乙醇提取物。研究表明,试验所用的玉米芯中多酚提取工艺稳定、可靠,可以为玉米芯多酚的高效提取提供参考。

The experiment was aimed to optimize the extraction technology of polyphenols from corn cob.The types of natural deep eutectic solvents for extraction were determined through screening experiments.Based on the results of single-factor experiments,the water content of the NADESs,liquid-to-solid ratio,ultrasonic power,and ultrasonic temperature were set as variables,with the extraction yield of corn cob polyphenols as the response value,and the response surface method was employed to optimize the extraction process.The scavenging abilities of polyphenol extracts from the NADESs and 70%ethanol on 1,1-diphenyl-2-trini-trophenylhydrazi-ne(DPPH)radicals and 2,2-diazodi bis(3-ethylbenzothia-zole-6-sulfonic acid)diamine(ABTS)radicals was compared.The results showed that at a NADESs water content of 31%,a liquid-to-solid ratio of 28 mL/g,an ultrasonic power of 350 W,and an ultrasonic temperature of 61℃,the extraction yield of corn cob polyphenols reached the highest level of 17.49 mg/g,and the antioxidant activity of the extracted product was stronger than that of the 70%ethanol extract.The study indicates that the extraction process of polyphenols from corn cob was stable and reliable,which can provide reference for the efficient extraction of polyphenols from corn cob.