广西新记录种井冈两头蛇的鉴定及其系统发育关系分析

Identification of a New Record Species of Calamaria jinggangensis in Guangxi,China, with Phylogenetic Relationship Analysis of the Species

井冈两头蛇Calamaria jinggangensis Cai,2023隶属游蛇科Colubridae,目前仅记录分布在江西井冈山。2021年9月至2022年6月,在广西桂林全州县才湾镇、龙胜县黄沙镇采集到2号疑似井冈两头蛇的样本(标本号GXNU20210909007和GXNU20220613012)。基于线粒体细胞色素b(cyt b)基因的系统发育分析表明,桂林采集的两头蛇样本与井冈两头蛇地模标本聚为一支,并且2号标本与井冈两头蛇的遗传距离为0.005~0.030,小于同属内其他物种之间的遗传距离(0.052~0.237)。经过形态学比较和系统发育分析,确认该2号标本为两头蛇属Calamaria的井冈两头蛇C.jinggangensis。此发现为该物种在广西的新记录,为井冈两头蛇的地理分布和系统发育提供更多的信息。

Calamaria jinggangensis Cai,2023,which belonging to the family of Colubridae,was only known to be distributed in Jinggang Mountains,Jiangxi,China.On September 3,2021 and June 2,2022,two specimens(No.GXNU20210909007 and GXNU20220613012)presented similar morphological features with C.jinggangensis were collected from Guilin,Guangxi Zhuang Autonomous Region.Phylogenetic analysis based on the mitochondrial cytochrome b(cyt b)gene showed that specimens from Guilin clustered into a single unit with the type specimen of C.jinggangensis.Genetic distance between the collected samples and C.jinggangensis was 0.005-0.030,significantly smaller than the genetic distance between other species within the genus Calamaria 0.052-0.237.Through morphological comparison and phylogenetic analysis,it is confirmed that the two specimens are C.jinggangensis which belongs to the genus Calamaria.This discovery is the first record of this species in Guangxi,which could provide more information on the geographic distribution and phylogeny of Calamaria species.