高效液相色谱法测定头孢曲松钠聚合物

High Performance Liquid Chromatography Determination of Ceftriaxone Sodium Polymer

目的:采用高效液相色谱法测定头孢曲松钠聚合物的含量,并进行方法验证。方法:色谱柱以十八烷基硅烷键合硅胶为填充剂,V(0.02 moL/L正辛胺溶液)∶V(乙腈)=73∶27,pH值(磷酸调节)6.5为流动相A,流动相B为乙腈,流速为1.0 mL/min,柱温为30℃,检测波长254 nm,采用梯度洗脱的方式进行实验。结果:该方法专属性、检测限和定量限、精密度、线性、耐用性等参数均符合要求。结论:该方法可用于测定头孢曲松钠聚合物含量,准确度和灵敏度高。

Objective:To determine the content of cephalosporin sodium polymer with high-efficiency liquid chromatography and verify the method.Method:The chromatography column uses ectumane silicane bonds silicone as the filling agent.V(0.02 mol/L positive amine solution)∶V(acetylene)=73∶27,pH value(regulating)6.5 is flow phase A,flow phase B is acetylene,the flow velocity is 1.0 mL/min,the column temperature is 30℃,the detection wavelength is 254 nm,and the experiment is conducted using gradient elution.Results:The specific attribute,detection limit and quantitative limit,precision,linear,durability and other parameters of this method meet the requirements.Conclusion:This method can be used to determine the content of spores and sodium polymer,high accuracy and sensitivity.