档案库房鞘翅目害虫DNA条形码鉴定研究

DNA Barcoding Identification of Archives Coleoptera Pests

鞘翅目害虫是目前档案库房中最常见的有害生物之一,害虫严重爆发时,被蛀食的档案纸张被大面积毁损且无法修复,所以档案虫情监测和预防极为重要。由于库房中害虫成虫采集难度大、害虫形态虫态差别大、发生规律各异、专业人员紧缺等,档案库房鞘翅目害虫的快速准确鉴定成为一个难点。为了更好地解决这个问题,本研究利用DNA条形码鉴定技术将采集到的样本进行分子鉴定,利用线粒体基因组的COI基因片段,建立了档案鞘翅目害虫DNA条形码数据库。除此之外,本研究还利用分子数据将三种害虫的成、幼虫进行配对,为解决幼虫形态鉴定困难的问题提供了新的思路。最后,本研究讨论了如何更好地结合形态数据和DNA条形码数据进行物种鉴定,说明了DNA条形码技术能利用最少的取样实现快速高效鉴定档案鞘翅目害虫常见种类各虫态样本的目标。

Coleopteran are one of the most common pests in the archive rooms.In the case of serious pest outbreaks,the archival papers eaten by pests are irreparably damaged,so it is really important to monitor the situation and prevent the burst of pests in archives.The rapid and accurate identification of coleopteran pests has become a hard case due to the difficulty of collecting pests,the obvious individual differences,the disordered occurrence rules,and the shortage of professional taxonomist.In order to solve this problem,This study used DNA barcoding technology to identify the archival pest samples based on the mitochondrial COI gene,established the archival coleopteran pest DNA barcode database.Beyond that,this study found 3 pairings between adult and larva,which may provide a new way to solve the problem of identifying larva.Finally,this study discussed how to better combine morphological data and DNA barcode data to identity species,illustrated that DNA barcoding technology is a qualified method that can help researchers to identify archival coleopteran pests.