核壳磁性胡桃醌分子印迹聚合物对核桃青皮中目标分子的选择性富集

Selective Enrichment of Juglone in Walnut Green Peel Using Core-Shell Magnetic Molecularly Imprinted Polymer

本实验利用表面印迹技术,以氨基功能化Fe_(3)O_(4)纳米粒子为磁核,按胡桃醌、功能单体、交联剂物质的量比为1∶8∶40,采用一步法制备核壳磁性胡桃醌分子印迹聚合物(molecularly imprinted polymer,MIP)(Fe_(3)O_(4)-NH_(2)@MIP)。通过扫描电镜、透射电镜、傅里叶变换红外光谱等多种手段对材料的形貌、结构、磁强度等进行表征;通过动力学吸附、等温吸附实验探究聚合物的吸附性能。结果表明,在Fe_(3)O_(4)-NH_(2)表面成功包裹厚度约为75 nm的印迹层,形状为类球状,具有规则的晶体结构,饱和磁化强度为48.50 emu/g,在外加磁场的作用下5 s迅速得以分离;当吸附时间100 min、胡桃醌质量浓度为100μg/m L时,吸附性能较好,吸附量为32.89μg/mg,动力学吸附过程更接近准二级动力学模型,吸附等温线符合Langmuir模型,说明Fe_(3)O_(4)-NH_(2)@MIP可以高效地从核桃青皮提取液中分离富集胡桃醌。本研究对胡桃醌更多生物活性的开发以及拓展其在食品、药业等行业的应用具有一定的参考作用。

In this study,surface imprinting technology was used for the one-step preparation of a core-shell magnetic molecularly imprinted polymer(Fe_(3)O_(4)-NH_(2)@MIP)with amino functionalized magnetic iron trioxide(Fe_(3)O_(4)-NH_(2))as the magnetic core at a mass ratio of juglone to functional monomer to cross-linking agent of 1:8:40.The structure,morphology and magnetic strength of the material were characterized by multiple techniques including scanning electron microscopy(SEM),transmission electron microscopy(TEM),and Fourier transform infrared spectroscopy(FTIR).The adsorption performance was studied through dynamic adsorption and isothermal adsorption experiments.Results showed that an imprinted layer with a thickness of about 75 nm was successfully polymerized on the surface of Fe_(3)O_(4)-NH_(2),with a nearly spherical shape and a regular crystal structure and a saturation magnetic strength of 48.50 emu/g.The layer could be quickly separated in five seconds.When the adsorption time was 100 minutes and the concentration of juglone was 100μg/mL,good adsorption performance was obtained with an adsorption capacity of 32.89μg/mg,the kinetic adsorption process was closer to a pseudo-second-order model,and the adsorption data accorded with the Langmuir isotherm,indicating that Fe_(3)O_(4)-NH_(2)@MIP enables efficient separation and enrichment of juglone from walnut green peel extract.This study is of reference significance for the development of more biological activity of juglone and the expansion of its application in food,pharmaceutical and other industries.