植物乳植杆菌NXU0011发酵枸杞产物对溃疡性结肠炎小鼠代谢物质的影响

Effects of Goji Berries(Lycium barbarum L.)Fermented by Lactobacillus plantarum NXU0011 on Serum Metabolites in Mice with Ulcerative Colitis

为探究植物乳植杆菌NXU0011发酵枸杞产物对溃疡性结肠炎小鼠代谢物质的影响,以葡聚糖硫酸钠(dextran sodium sulfate,DSS)诱导的溃疡性结肠炎模型小鼠为研究对象,将其分为模型组(DSS组)、空白组(Con组)、药物组(MS组)、植物乳植杆菌NXU0011发酵枸杞产物冻干粉干预组(LP+Ly组),基于非靶向代谢组学技术,对其血清进行差异代谢物质分析。在正离子模式下,鉴定出4组样本间共有381种差异代谢物,其中MS、DSS比较组样本间差异代谢物有56种,LP+Ly、DSS比较组样本间差异代谢物有87种。对Con组和LP+Ly组与DSS组显著差异代谢物进行京都基因与基因组百科全书富集分析,筛选出精氨酸生物合成、维生素消化和吸收两条富集率较高的代谢途径。本研究可为乳酸菌发酵枸杞产物改善溃疡性结肠炎提供参考依据。

This study was carried out in order to investigate the effects of goji berries fermented by Lactobacillus plantarum NXU0011 on serum metabolites in mice with ulcerative colitis(UC).A mouse model of UC was induced by oral administration of dextran sodium sulfate(DSS)in drinking water.Four groups of mice were set up:DSS-induced model,control(Con),medication with mesalazine(MS),and intervention with lyophilized powder of L.plantarum NXU0011-fermented L.barbarum L.(LP+Ly).Non-targeted metabolomics was used to analyze differential serum metabolites among the four groups.In the positive ion mode,a total of 381 differential metabolites were identified,including 56 differential metabolites between the MS and DSS groups and 87 differential metabolites between the LP+Ly and DSS groups.Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis of the significantly differential metabolites between the Con,LP+Ly and DSS groups revealed two metabolic pathways with the higher enrichment rates,arginine biosynthesis and vitamin digestion and absorption.This study provides a reference for the application of Lactobacillus-fermented goji berries to ameliorate UC.