高压氧联合乌司他丁对急性有机磷农药中毒大鼠胆碱乙酰转移酶、丙二醛及心肌功能的影响

Effects of hyperbaric oxygen combined with ulinastatin on choline acetyltransferase,malondialdehyde,and myocardial function in rats with acute organophosphorus pesticide poisoning

目的探究高压氧联合乌司他丁对急性有机磷农药中毒大鼠胆碱乙酰转移酶(choline acetyltransferase,ChAT)、丙二醛(malondialdehyde,MDA)及心肌功能的影响。方法选取50只无特定病原体(specific-pathogen-free,SPF)级SD雄性大鼠,随机分为正常组(N组)、模型组(M组)、高压氧组(H组)、乌司他丁组(U组)、高压氧联合乌司他丁组(O组),每组10只,对M、H、U、O组采用颈背部皮下累积注射法建立急性有机磷农药中毒模型,N组不建立该模型,建模成功后,对H组给与高压氧治疗,对U组腹腔注射乌司他丁10万U/kg,对O组给与高压氧联合乌司他丁治疗,N组、M组同期给与灌胃同体积生理盐水,心脏彩超检测大鼠心肌功能,HE染色法及尼氏染色法检测大鼠脑组织病理形态,TUNEL法检测心肌细胞凋亡,光化学法检测大鼠脑组织中ChAT、乙酰胆碱(acetylcholine,Ach)含量,硫代巴比妥酸比色法检测大鼠血清中MDA、超氧化物歧化酶(superoxide dismutase,SOD)水平,采用SPSS 22.0软件进行统计学分析。结果与N组相比,M组左室收缩末期内径(left ventricular end systolic dimension,LVSD)与左室舒张末期内径(left ventricular end diastolic dimension,LVDD)均有所扩大,室间隔(interventricular septum,IVS)厚度增加,左心室劳损(left ventricular strain,LVS)加剧,心肌细胞凋亡数量增多,Ach与MDA含量均上升(P<0.05),左室射血分数(Left ventricular ejection fraction,LVEF)降低,ChAT与SOD含量均降低(P<0.05);与M组比较,H、U两组大鼠LVSD与LVDD均有所缩小,IVS厚度减少,LVS降低,心肌细胞凋亡数量减少,Ach与MDA含量均降低(P<0.05),LVEF升高、ChAT与SOD含量升高(P<0.05),U组与H组相比差异无统计学意义(P>0.05),O组比U组变化明显(P<0.05);N组大鼠脑组织病理形态正常,M组脑组织结构损伤严重,与M组相比,H、U、O组症状明显改善。结论高压氧联合乌司他丁对急性有机磷农药中毒大鼠具有显著疗效,可显著升高ChAT含量、降低MDA含量,并有效改善心肌功能。

Objective To investigate the effects of hyperbaric oxygen combined with ulinastatin on choline acetyltransferase(ChAT),malondialdehyde(MDA),and myocardial function in rats with acute organophosphorus pesticide poisoning.Methods 50 specific-pathogen-free(SPF)male SD rats were randomly divided into five groups of ten each:normal group(N),model group(M),hyperbaric oxygen group(H),and ulinastatin group(U),and hyperbaric oxygen combined ulinastatin(O)group.The model of acute organophosphorus pesticide poisoning was established in the M,H,U,and O groups via cumulative subcutaneous injections on the neck back,and the N group did not set up the model.After the success of the modeling,the H group was treated with hyperbaric oxygen therapy,and the U group was intraperitoneally injected with ulinastatin at a dose of 100000 U/kg.The O group received a combination of hyperbaric oxygen and ulinastatin treatment,whereas the N and M groups were gavaged with an equivalent volume of saline.The rats'myocardial function was assessed through cardiac echocardiography,brain tissue pathology was examined using hematoxylin and eosin(HE)staining and Nissl staining,cardiomyocyte apoptosis was evaluated by TUNEL assay,and ChAT and acetylcholine(Ach)content in rat brain tissues were determined through histochemical methods.Serum levels of MDA and superoxide dismutase(SOD)were measured using the thiobarbituric acid reactive substances(TBARS)assay.Statistical analysis was performed by SPSS 22.0.Results Compared with Group N,the left ventricular end systolic dimension(LVSD)and left ventricular end diastolic dimension(LVDD)in Group M were both enlarged,with increased interventricular septum(IVS)thickness,aggravated left ventricular strain(LVS),increased number of apoptotic cardiomyocytes,and elevated Ach and MDA levels(P<0.05).Additionally,the left ventricular ejection fraction(LVEF)decreased,while ChAT and SOD levels also decreased(P<0.05).When compared to Group M,both Groups H and U exhibited reduced LVSD and LVDD,thinner IVS,decreased LVS,and fewer apoptotic cardiomyocytes.Furthermore,Ach and MDA levels were lower(P<0.05),while LVEF,ChAT,and SOD levels were higher(P<0.05).No statistically significant difference was observed between Groups U and H(P>0.05),while Group O showed more pronounced changes compared to Group U(P<0.05).Rats in Group N exhibited normal brain tissue pathological morphology,while those in Group M suffered severe damage to brain tissue structure.Compared to Group M,significant improvements in symptoms were observed in Groups H,U,and O.Conclusions Hyperbaric oxygen combined with ulinastatin has a significant effect on acute organophosphorus pesticide poisoning rats,which can significantly increase ChAT content,decrease MDA content,and effectively improve myocardial function.