链荚豆不同提取物体外抑石及抗氧化作用研究

Study on Inhibition Effect of Calcium Oxalate Stone and Antioxidant Activity by the Different Extract from Alysicarpus vaginalis(L.)DC.in vitro

目的研究探讨链荚豆不同提取物对草酸钙结石的抑制作用及抗氧化作用。方法采用扫描电镜、X射线衍射、傅里叶红外光谱、Zeta电位分析等方法对添加链荚豆不同提取物形成的草酸钙晶体进行表征;以维生素C为阳性对照,研究链荚豆不同提取物对羟基自由基的清除率。结果添加链荚豆不同提取物后形成的草酸钙晶体,通过SEM观察多呈四角椎形,主要以COD晶体形式存在;通过XRD和FT-IR测定分析,COD百分含量均高于无添加链荚豆提取物的草酸钙标准品;通过测定晶体表面Zeta电位,随着COD百分含量增高,其Zeta电位的绝对值增大,晶体的分散度增加。与阳性对照V c比较,链荚豆不同提取物均具有清除羟基自由基的能力(P<0.05)。结论链荚豆不同提取物不但具有抑制草酸钙晶体聚集成核,诱导COD生长,而且具有抗氧化作用,有利于抑制草酸钙结石的形成。

OBJECTIVE To study the inhibitory and antioxidant activity of different extracts from Alysicarpus vaginalis(L.)DC on calcium oxalate stones.METHODS Scanning electron microscope,X-ray diffraction,Fourier transform infrared spectroscopy,nanometer particle size analyzer and other methods were used to characterize the calcium oxalate crystals formed by adding different extracts of Alternaria pedunculata L;Using vitamin C as a control,study the clearance rate of hydroxyl radicals by different extracts from Alysicarpus vaginalis(L.)DC.RESULTS The calcium oxalate crystals formed by adding different extracts from Alysicarpus vaginalis(L.)DC were mostly in the form of quadrangular pyramids,mainly in the form of COD crystals;By XRD and FT-IR analysis,the percentage content of COD was higher than that of the standard calcium oxalate;By measuring the Zeta potential on the crystal surface,as the COD content increases,the absolute value of the Zeta potential increases and the dispersion of the crystal increases.Compared with the positive control Vc,different extracts of Scutellaria japonica have the ability to scavenge hydroxyl radicals(P<0.05).CONCLUSION The different extracts from Alysicarpus vaginalis(L.)DC not only inhibit the nucleation of calcium oxalate crystals and induce the growth of COD,but also have antioxidant effects,which is conducive to inhibiting the formation of calcium oxalate stones.