转录因子ZBTB20对垂体瘤侵袭发展的影响

Effects of transcription factor ZBTB20 on PitNET invasive progression

目的研究转录因子ZBTB20在垂体瘤侵袭发展中的作用。方法首先通过免疫组织化学染色检测临床肿瘤样本中ZBTB20的表达情况;随后通过转染ZBTB20和sh-ZBTB20慢病毒建立ZBTB20过表达和敲减的GH3垂体瘤细胞系;通过CCK-8细胞增殖实验、划痕愈合实验检测ZBTB20对垂体瘤细胞增殖、侵袭能力的影响;同时通过蛋白免疫印迹(WB)、实时荧光定量PCR(qPCR)检测增殖侵袭相关基因表达的改变。结果免疫组织化学染色结果显示,侵袭性垂体瘤样本中ZBTB20表达量显著高于非侵袭性样本;ZBTB20过表达及敲减的GH3细胞系研究中,CCK-8细胞增殖实验、划痕愈合实验结果提示ZBTB20提高了GH3细胞的增殖能力和侵袭能力,WB和qPCR结果显示该作用与PCNA、MMP2、MMP14蛋白表达增加有关。结论转录因子ZBTB20与垂体瘤侵袭发展呈正相关,ZBTB20通过促进PCNA、MMP蛋白表达提高垂体瘤细胞增殖、侵袭能力,对深入理解垂体瘤侵袭发展机制、发掘潜在治疗靶点有重要意义。

Objective To study the effects of the transcription factor ZBTB20 on the invasive progression of pituitary neuroendocrine tumors(PitNET).Methods Tumor sample slices were stained by using immunohistochemical(IHC)method,and ZBTB20 expression levels were measured in these tissues;ZBTB20 overexpressed/knock-down cell lines were established by transfecting ZBTB20/sh-ZBTB20 lentivirus into GH3 cell line;CCK-8 proliferation assay and wound-healing assay were used to measure the effects of ZBTB20 on GH3 cell proliferation and migration;WB and qPCR were used to measure the expression change of proliferation,migration and invasion related to genes.Results IHC staining results showed that ZBTB20 expression was significantly higher in invasive PitNET samples than that in noninvasive samples;in the study of ZBTB20 overexpressed/Knock-down cell lines,CCK-8 proliferation assay and wound-healing assay suggested that ZBTB20 improved the proliferation ability and migration ability of GH3 cell,which was correlated with upregulation of PCNA,MMP2 and MMP14 expression,as shown by WB and qPCR.Conclusion The transcription factor of ZBTB20 is positively correlated with the invasion and development of pituitary tumors.ZBTB20 enhances the proliferation and invasion ability of pituitary tumor cells by promoting the expression of PCNA and MMP proteins,which is of great significance for understanding the mechanism of invasion and development of pituitary tumors and exploring potential therapeutic targets.