植物蛋白的亚细胞定位观察虚拟仿真实验

Virtual Simulation Experiment of Subcellular LocalizationObservation of Plant Protein

为实现实验教学数字化建设与生物学拔尖创新人才培养的有效融合,解决植物蛋白的亚细胞定位观察传统实验存在的实验成本高、精密仪器台套数不够、学生动手能力不足等问题,利用虚拟仿真技术开发了中英双语教学的植物蛋白的亚细胞定位观察虚拟仿真实验。该虚拟实验教学资源涵盖了拟南芥原生质体的制备、聚乙二醇介导的原生质体转化、荧光显微镜观察分析荧光蛋白定位等实验内容,打造了场景化、沉浸式与交互式相结合的实验教学新模式。通过在本科实验教学中的应用,加深了学生对蛋白质功能研究实验技术的认识,完善了普通生物学实验课程体系,实现了科研实训与实验教学的融合,培养了学生的创新能力、科研素养和国际竞争力,是传统实验教学的有效补充。

In order to realize the effective integration of the digital construction of experimental teaching and the cultivation of top innovative talents in biology,and solve the problems of the traditional experiment of the subcellular localization observation of plant protein,such as high experiment cost,insufficient number of precision instrument sets and insufficient practical ability of students,the virtual simulation technology is used to develop the virtual simulation experiment of subcellular localization observation of plant protein in Chinese and English bilingual teaching.This virtual experimental teaching resource covers the preparation of Arabidopsis protoplast,protoplast transformation mediated by polyethylene glycol,fluorescence microscope observation and analysis of fluorescent protein localization and other experimental contents,creating a new experimental teaching model that combines scene,immersion and interaction.Through its application in undergraduate experimental teaching,it deepens students’understanding of the protein function research experimental technology,improves the curriculum system of the general biology experiment,realizes the integration of scientific research practical training and experimental teaching,and cultivates students’innovative ability,scientific research accomplishment and international competitiveness,which is an effective supplement to the traditional experimental teaching.