单宁酸交联明胶微球的制备及载药性能研究

Study on Preparation and Drug-Loading Properties of Tannic Acid-Crosslinked Gelatin Microspheres

目的:以天然植物提取物单宁酸为交联剂,明胶为原料,采用乳化交联法制备单宁酸明胶微球,并对其制备工艺进行优化和评价。方法:以外观形貌、平均粒径为指标,通过单因素试验筛选结合正交设计试验优化单宁酸明胶微球的制备工艺,最终制备花青素装载的单宁酸明胶微球;采用pH值示差法测定明胶微球的药物包封率及载药量。结果:单宁酸交联的明胶载药微球成球性好、形态圆整,大小均匀,优化后的微球平均粒径为(74.83±1.16)μm,在酸性、碱性环境下具有一定的耐腐蚀性。同时载药明胶微球的包封率可达89.90%,载药量为3.68%。结论:经单宁酸交联的明胶微球形态好、粒径适宜,并具有较高的药物装载率,为单宁酸交联明胶微球的后续研究提供了实验基础。

Objective:In this paper,tannic acid gelatin microspheres were prepared by an emulsification-crosslinking method with natural plant extract tannic acid as crosslinking agent and gelatin as raw material,and the preparation process was optimized and evaluated.Methods:Taking appearance morphology and average particle size as indexes,the preparation process of tannic acid gelatin microspheres was optimized by single-factortests screening combined with orthogonal design experiment,and finally the tannic acid gelatin microspheres loaded with anthocyanins were prepared.The drug encapsulation rate and drug load of gelatin microspheres were determined by pH differential method.Results:Tannic acid cross-linked gelatin microspheres have good pelletization,rounded shape and uniform size.The optimized microspheres have an average particle size of(74.83±1.16)μm.At the same time,the encapsulation rate and drug loading of gelatin microspheres were up to 89.90%and 3.68%respectively.Conclusion:Gelatin microspheres crosslinked by tannic acid have good morphology,suitable particle size and high drug loading rate,which provide experimental basis for the follow-up research of tannic acid crosslinked gelatin microspheres.