UPLC-MS/MS法同时测定化妆品中47种抗感染类药物

Simultaneous Determination of 47 Anti-infective Drugs in Cosmetics by UPLC-MS/MS

建立超高效液相色谱串联质谱法(UPLC-MS/MS)同时测定三种基质(水剂、乳剂、膏霜)化妆品中47种抗感染类药物的分析方法。样品加饱和氯化钠使分散,经甲醇超声提取后上机检测,各组分以基质标准曲线外标法定量。结果47种抗感染类药物在各自线性浓度范围内线性关系良好,检出限为0.02~0.47 mg/kg,定量限为0.10~2.37 mg/kg,3个加标水平平均回收率为80.7%~119.8%,相对标准偏差(RSD)均小于10%。本法具有预处理简便,响应度高,准确可靠等优点,可用于化妆品中47种抗感染类药物快速准确地定性筛查与定量测定。

To simultaneous determine 47 kinds of anti-infective drugs in cosmetics with three matrix types(water,emulsion,cream)by ultra high performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).The sample was dispersed by saturated sodium chloride,and then was ultrasonically extracted with methanol.And each component was quantified by an external standard curve with matrix.The results:47 kinds of anti-infective drugs showed good linear relationships in their respective linear concentration ranges.The limits of detection(LODs)were 0.02~0.47μg/kg,and the limits of quantitation(LOQs)were 0.10~2.37μg/kg.The average recoveries at three spiked levels were 80.7%~119.8%,with the relative standard deviations(RSD)not greater than 10.0%.This method has the advantages of simple pretreatment,high response,accuracy and reliability.It can be used for the rapid and accurate qualitative screening and quantitative determination of 47 anti-infective drugs in cosmetics.