不同湿法消解体系-原子吸收分光光度法测定灵芝中铁含量

Determination of Iron Content in Ganodermalucidum by Atomic Absorption Spectrophotometry with Different Wet Digestion Systems

探究不同消解体系对灵芝中Fe消解的影响,构建混酸为消解体系的前处理对灵芝中Fe含量的检测方法。采用原子吸收分光光度法测定Fe的含量;以硝酸-高氯酸为基础进行对灵芝子实体的消解,以Fe的含量为指标,优化不同类型、不同体积比的混酸消解体系,对紫芝和赤芝进行湿法消解,考察最优的混酸体系对灵芝中Fe含量测定的消解效果。研究结果表明,在质量浓度为2.00~10.00μg/L范围内,Fe标准工作曲线的方程为:Y=0.0176X+0.0053,R^(2)=0.9994;在混酸体积比例为V(HNO_(3))∶V(HClO_(4))∶V(H_(2)O_(2))=8∶1∶2的体系下,测得紫芝中Fe含量为90.32μg/g,赤芝中Fe含量为86.25μg/g,RSD分别为2.43%和2.18%。

Toconstruct a mixed acid digestion systemfor determingFe content in Ganoderma lucidum.The content of Fewas determinedusing atomicabsorption spectrophotometrymethod.The digestion ofGanodermalucidumfruitbody is based on nitric acid-Perchloric acid,with the Fecontent as the indicator,themixed acid digestion systems of different types and volume ratioswre optimized,and wet digestion of Ganodermalucidum and Ganodermalucidum to obtain the optimal mixed acid system for the digestion of Fe.According to the experimental results,at a concentration of 2.00μg/L to 10.00μg/L,theformulaof Festandard working curveis Y=0.0176X+0.0053,R 2=0.9994.In the system with a mixed acid volume ratio of V(HNO_(3))∶V(HClO_(4))∶V(H_(2)O_(2))=8∶1∶2,the Fecontent inGanodermasinenseis 90.32μg/g,and 86.25μg/g in Ganodermalucidum,the RSD is 2.43%and 2.18%respectively.