超声辅助低共熔溶剂葛根多糖提取工艺及其抗氧化活性研究

Ultrasonic-assisted Deep Eutectic Solvent Extraction Process and Antioxidant Activity of Pueraria Lobata Polysaccharides

本文采用超声辅助低共熔溶剂对葛根多糖的最佳提取工艺及其抗氧化活性展开研究。以葛根为原料、超声辅助低共熔溶剂为提取方法,采用单因素和Box-Behnken法优化葛根多糖的提取工艺,并用DPPH法对葛根多糖的抗氧化活性进行研究。通过对低共熔溶剂的筛选,确定了以氯化胆碱和乙二醇1∶2配置作为最佳提取溶剂配比,优选葛根多糖的最佳提取工艺为:提取时间35min,提取温度40℃,液料比值14 m L/g,葛根多糖的平均提取率为13.27%。在葛根多糖的质量浓度为1.00 mg/mL时对DPPH自由基的清除率最高,可达88.72%。超声辅助低共熔溶剂提取葛根多糖提取率高、绿色环保,且能较好地保存目标产物的活性。

This paper researched the optimal extraction process of Pueraria lobata polysaccharides by using ultrasonic-assisted deep eutectic solvent and the antioxidant activity of Pueraria lobata polysaccharides.Taking the Pueraria as the raw material and the ultrasonic-assisted deep eutectic solvent as the extraction method,the extraction process of Pueraria lobata polysaccharides was optimized by single factor and Box-Behnken method,and the antioxidant activity of Pueraria lobata polysaccharides was studied by DPPH method.Through the optimization of deep eutectic solvents,choline chloride-ethylene glycol 1∶2 was determined as the optimal extraction solution,detailed as follows:extraction time 35 min,extraction temperature 40℃,liquid-material ratio 14 mL/g,and the average extraction rate of Pueraria lobata polysaccharides was 13.27%.When the concentration of Pueraria lobata polysaccharides was 1.00 mg/mL,the highest scavenging rate of DPPH free radicals was up to 88.72%.The ultrasonic-assisted deep eutectic solvent extraction of Pueraria lobata polysaccharides had a high extraction rate,was environmentally friendly,and could preserve the activity of the target product.