嗜碱芽孢杆菌细胞破碎方法比较及其功效成分分析

Comparison of Cell Disruption Methods of Bacillus alkaliphilic and Analysis of Its Functional Components

极端微生物是最适合生活在极端环境中的微生物的统称,体内富含多种功效成分,在化妆品领域具有相当广阔的应用前景,所以获取极端微生物中的活性成分十分重要。对此,文章以开发极端微生物中的活性成分为目的,以期为嗜碱微生物在化妆品领域的开发和利用提供可靠的依据。研究选用嗜碱芽孢杆菌进行单因素试验以确定其培养条件,通过三种常见的破碎方法确定嗜碱芽孢杆菌的最佳破碎方式,然后测定溶胞物中的功效成分并评估相应成分的抗氧化功效。试验结果表明,嗜碱芽孢杆菌的最佳培养条件为接种量3%,培养温度30℃,培养时间26 h,转速180 r/min。通过对比三种常用的破碎法得出,高压细胞破碎法的效果最佳,破碎后的菌体浓度最低,得到的蛋白质含量最高。此外,研究还测定了溶胞物中活性物质的含量,并进行了抗氧化效果评估。结果表明,溶胞物中多糖、多酚和多肽的含量分别为2.43 mg/mL、6.27 mg/mL和0.607 mg/mL。嗜碱芽孢杆菌对DPPH自由基、ABTS自由基的清除率随着溶胞物质量分数的升高而逐渐增强,当质量分数为20%时能够达到较好的效果,此时DPPH自由基、ABTS自由基的清除率分别为43.3%、37%,铁原子还原能力为33.9%。

Extreme microorganisms are a collective term for microorganisms that are most suitable for living in extreme environments,which are rich in a variety of functional components and have broad application prospects in the field of cosmetics.Therefore,it is very important to obtain the active components of extreme microorganisms.This paper aims to develop the active components of extreme microorganisms,in order to provide reliable basis for the development and utilization of alkaliphilic microorganisms in the field of cosmetics.In this experiment,alkaliphilic microorganisms were used to determine the culture conditions by single factor experiment.By using three common disruption methods,the best disruption method of Bacillus alkaliphilic was determined,the functional components of lysing substances were determined,and the antioxidant effect of related components was determined.The experimental results showed that the optimum culture conditions of Bacillus alkaliphilic were as follows:inoculation amount of 3%,culture temperature of 30℃,culture time of 26 h,and rotation speed of 180 r/min.By comparing the three common disruption methods,it was concluded that the high-pressure cell disruption method has the best effect,the cell concentration after crushing was the minimum,and the obtained protein content was the highest.In addition,the content of the functional components in the lysosomal substance was determined and the antioxidant effect was determined.The results showed that the contents of polysaccharides,polyphenols and peptides in the lysosomal substance were respectively 2.43 mg/mL,6.27 mg/mL and 0.607 mg/mL.With the increase of the mass fraction of lysosomal substance,the clearance rate of DPPH and ABTS free radicals by Bacillus alkaliphilic gradually increased.The better effect was achieved when the volume fraction was 20%,with the DPPH free radical and ABTS free radical scavenging rates of 43.3%and 37%respectively,and the reducing ability of 33.9%.