CYB561在肝细胞癌中的表达及其临床意义

Expression of CYB561 in hepatocellular carcinoma and its clinical significance

目的探究细胞色素b561(cytochromes b561,CYB561)在肝细胞癌(hepatocellular carcinoma,HCC)组织中的表达及其临床意义。方法通过数据库资料分析CYB561 mRNA在HCC组织中的表达情况及其与患者预后的关系。采用免疫组织化学(immunohistochemistry,IHC)法检测CYB561蛋白在61例配对HCC组织及其癌旁组织中的表达,分析CYB561蛋白表达与HCC患者的临床病理特征及预后的关系。采用KaplanMeier法绘制生存曲线,采用Cox比例风险回归模型分析CYB561蛋白表达与预后的相关性。结果数据库资料分析结果显示:CYB561 mRNA在HCC组织中的相对表达量高于癌旁组织(P<0.001);与CYB561 mRNA表达阴性HCC患者相比,CYB561 mRNA表达阳性HCC患者的总生存期(overall survival,OS)、无复发生存期、无进展生存期和无病生存期更差(均P<0.05)。IHC结果显示,CYB561蛋白在HCC组织和癌旁组织中的表达阳性率分别为57.38%(35/61)和21.31%(13/61),前者高于后者,差异有统计学意义(χ^(2)=16.624,P<0.001);生存分析结果显示,CYB561蛋白表达阳性患者的OS差于表达阴性患者(P<0.05);多因素Cox比例风险回归分析结果显示,CYB561蛋白阳性表达是HCC患者术后OS的危险因素[HR=3.308,95%CI为(1.344,8.144),P=0.009]。结论CYB561在HCC中呈阳性表达且提示更差的生存预后,可作为HCC的潜在预后生物学标志物。

Objective To investigate the expression and clinical significance of cytochromes b561(CYB561)in hepatocellular carcinoma(HCC).Methods The expression of CYB561 mRNA in HCC tissues and its relationship with prognosis were analyzed by database data.Immunohistochemistry(IHC)was used to detect the expression of CYB561 protein in 61 matched HCC tissues and their adjacent tissues,and the relationship between CYB561 protein expression and clinicopathological features and prognosis of HCC was analyzed.Kaplan-Meier method was used to draw the survival curve and Cox proportional hazard regression model was used to analyze the correlation between the expression of CYB561 protein and the prognosis of HCC.Results The analysis of database data showed that the relative expression of CYB561 mRNA in HCC tissues was higher than that in adjacent tissues(P<0.001).Compared with HCC patients with negative expression of CYB561 mRNA,HCC patients with positive expression of CYB561 mRNA had worse overall survival(OS),relapse-free survival,progression-free survival and disease-free survival(all P<0.05).The results of IHC showed that the positive rates of CYB561 protein in HCC tissues and adjacent tissues were 57.38%(35/61)and 21.31%(13/61),respectively.The former was higher than the latter,with statistical significance(χ^(2)=16.624,P<0.001).Survival analysis showed that the OS of patients with positive expression of CYB561 protein was worse than that of patients with negative expression(P<0.05).Multivariate Cox proportional hazard regression analysis showed that the positive expression of CYB561 protein was a risk factor for postoperative OS in HCC patients[HR=3.308,95%CI(1.344,8.144),P=0.009].Conclusion CYB561 is positively expressed in HCC and suggests a worse survival,and may serve as a potential prognostic biomarker for HCC.