巨大侧耳ABC转运蛋白家族的全基因组分析及其对镉离子胁迫的响应

Genome-wide analysis and the response to cadmium ion stress of ABC transporter family in Pleurotus giganteus

腺苷三磷酸结合盒转运蛋白(ATP-binding cassette transporter,ABC转运蛋白),广泛存在于所有生物中,是目前研究最多且最大的蛋白家族之一。该蛋白家族部分成员具有跨膜转运重金属离子和应对非生物胁迫的作用。本研究利用Blastp和Hmmer软件在全基因组水平筛选巨大侧耳的ABC转运蛋白家族成员,并对其进行生物信息学分析,然后依据转录组数据筛选差异基因,并对该家族编码基因整体表达趋势进行分析。总计获得38个巨大侧耳ABC转运蛋白基因,可分为G、B、C、F、I、D、A和E共8个亚家族,分别有6、5、10、3、8、3、2和1个成员。其主要分布在各细胞器膜上,不同亚家族成员在结构域的种类和基序数量有区别,相同亚家族成员则具有较大的相似性。在镉离子胁迫的前期D亚族部分成员上调表达,胁迫中期C、D、I等亚族部分成员上调表达,后期B、C亚族部分成员上调表达。本研究表明部分ABC转运蛋白参与了巨大侧耳应对镉离子胁迫的反应,这些结果为进一步研究ABC转运蛋白家族在巨大侧耳在镉离子胁迫下发挥的作用及其分子机制提供了理论基础。

Adenosine triphosphate-binding cassette transporter(ABC transporter)proteins widely found in all organisms are one of the largest and extensively studied protein families.Some members of this protein family play an important role in transmembrane transport of heavy metal ions and in response to abiotic stresses.In this paper,Blastp and Hmmer software were used to screen and analyze bioinformatically the members of ABC transporter protein family in giant sidearm at the genome-wide level.Differential genes were screened on the basis of transcriptome data and the overall expression trend of genes encoding this family was analyzed.In total,38 ABC transporter protein genes of Pleurotus giganteus were obtained,which could be categorized into eight subfamilies,G,B,C,F,I,D,A and E,with 6,5,10,3,8,3,2 and 1 members,respectively.They are mainly distributed in the membranes of each cell organelle,with different subfamily members differing in the types of structural domains and the number of motifs,while members of the same subfamily have greater similarity.Some members of subfamily D were up-regulated in the early stage of cadmium ion stress,some members of subfamilies C,D and I were up-regulated in the middle stage of stress,and some members of subfamilies B and C were up-regulated in the late stage.The present study showed that some ABC transporter proteins were involved in the response of Cd ion stress in Pleurotus giganteus.These results provide a theoretical basis for further research on the role of ABC transporter protein family in Pleurotus giganteus and its molecular mechanism under Cd ion stress.