利用中华猕猴桃杂交后代转录组测序筛选抗溃疡病相关基因

Screening of Kiwifruit Canker Resistance-Related Genes Based on the Transcriptome Sequencing Analysis of Hybrids from Actinidia chinensis

猕猴桃细菌性溃疡病是猕猴桃产业中的毁灭性病害。不同遗传背景的猕猴桃材料抗性差异极大。为研究猕猴桃响应溃疡病菌(Pseudomonas syringae pv.actinidiae,Psa)的早期反应,挖掘抗病相关基因,对接种溃疡病菌6和24h后的中华猕猴桃‘金农’×中华猕猴桃雄株杂交后代中的抗病个体‘21-2-4’及感病个体‘19-3-11’进行转录组测序比较分析。结果表明抗病个体对Psa防御主要集中在24 h,而感病个体主要集中在6 h。KEGG通路分析表明差异表达基因主要富集于植物—病原菌相互作用、苯丙烷生物合成、淀粉和蔗糖代谢途径等初生、次生产物的代谢。对比抗、感病个体中的基因表达模式,对植物—病原菌相互作用、植物激素信号转导及苯丙烷生物合成等抗病相关途径中可能的候选基因进行初步筛选。以中华猕猴桃抗性品种‘金农’和感病品种‘红昇’为材料,通过qRT-PCR验证了初步筛选出来的基因,确定PR1(Acc06864/Acc06865/Acc06866)、FLS2(Acc06484)可作为抗病候选基因深入研究。

Kiwifruit bacterial canker is a devastating disease in the kiwifruit industry,causing huge losses to the industry.Great differences in resistance are found in materials with different genetic background.To study the early response of kiwifruit to canker and to explore the genes related to resistance,transcriptome sequencing and comparative analysis was performed on the resistant‘21-2-4’and the susceptible‘19-3-11’in the offspring of Actinidia chinensis‘Jinnong’×male after 6 h and 24 h inoculation with Pseudomonas syringae pv.actinidiae(Psa).The results showed that the defense of‘21-2-4’and‘19-3-11’against Psa mainly focused on 24 h and 6 h respectively.KEGG analysis showed that DEGs were mainly enriched in the primary and secondary metabolic pathways such as plant-pathogen interaction,phenylpropanoid biosynthesis,starch and sucrose metabolism.Gene expression patterns in resistant and susceptible materials were compared,and possible candidate genes in disease-esistant pathways such as plant-pathogen interaction,plant hormone signal transduction,and phenylpropane biosynthesis were screened.By using the resistant‘Jinnong’and susceptible‘Hongsheng’,the genes screened were verified by qRT-PCR,and then PR1(Acc06864/Acc06865/Acc06866)and FLS2(Acc06484)were selected as candidate genes for further study.