分蘖洋葱伴生番茄诱导的根际微生物对根结线虫病的影响

Effect of Rhizosphere Microorganisms Induced by Potato-Onion on Tomato Root-Knot Nematode Disease

采用16S rRNA高通量测序技术以及植物—土壤反馈方法,探究了分蘖洋葱伴生番茄诱导的根际微生物对其根结线虫病发生、土壤微生物群落结构以及诱导抗性的影响。结果表明,分蘖洋葱伴生的番茄根际微生物显著降低了番茄根结线虫的病情指数、根结数和根结指数,显著提高了对根结线虫卵孵化的抑制率和二龄幼虫的致死率。在根结线虫胁迫下,伴生增加了番茄根际潜在有益菌群芽孢杆菌属和假单胞菌属的相对丰度。同时,伴生改变的番茄根际微生物通过提高抗性相关基因表达、防御酶活性和茉莉酸类物质含量增强了番茄的系统抗性。因此,分蘖洋葱伴生改变的番茄根际微生物组除对根结线虫具有直接抑杀作用外,还通过对番茄产生诱导抗性提高其对根结线虫的抗病能力。

High-throughput sequencing and plant-soil feedback were used in this study,the effect of potato-onion companion induced tomato rhizosphere microorganisms on the tomato toot-knot nematode disease,soil microbial community structure and induced resistance were studied.The results showed that potato-onion companion induce the tomato rhizosphere microorganisms to decreased the tomato disease index,root-knot number and root-knot index and increased the Meloidogyne incognita egg hatching and the lethality rate.Under root-knot nematode stress,the Bacillus and Pseudomonas relative abundance were increased in tomato rhizosphere.Meanwhile,potato-onion companion changes tomato rhizosphere microbes enhanced systemic resistance by increasing expression of resistance-related genes,defense enzyme activity and JAs activity.In conclusion,potato-onion companion with tomato changed tomato rhizosphere microorganisms not only has direct inhibition effect on root-knot nematode,but also enhances resistance to root-knot nematode by inducing resistance.