单细胞测序技术在中枢神经系统肿瘤诊治中的应用

The application of the diagnosis and therapy of single cell sequencing technology in central nervous system tumors

中枢神经系统(CNS)肿瘤起因复杂,预后极差,肿瘤异质性是治疗失败和复发的主要原因,深入了解CNS肿瘤的生物多样性至关重要。单细胞测序技术为揭示CNS肿瘤复杂的生态系统提供了契机。本研究从肿瘤干细胞、肿瘤微环境以及脑脊液三个方面阐述了单细胞测序对CNS肿瘤异质性的探索,以及临床诊断、治疗及预后的意义。尽管大多数研究成果尚未在临床上应用,但为CNS肿瘤新临床指南的制定打下基础,以改善患者预后,预防肿瘤复发。

Central nervous system(CNS)tumors have complex causes and poor prognosis.Tumor heterogeneity is a major cause of treatment failure,and in-depth understanding of the biodiversity of CNS tumors is critical.Single-cell sequencing technology provides an opportunity to reveal the complex ecosystem of CNS tumors.In this study,we review the significance of single-cell sequencing in exploring the heterogeneity,diagnosis,treatment,and prognosis of CNS tumors from three aspects:tumor stem cells,tumor microenvironment,and cerebrospinal fluid.Although most of the findings have not been clinically applicable,they lay the foundation for the development of new guidelines for CNS tumors that help improve tumor prognosis and prevent tumor recurrence.