COL7A1的上调与肺腺癌的预后不良和免疫细胞浸润的生物信息学分析

Bioinformatics analysis of upregulated COL7A1 in lung adenocarcinoma:prognosis and immune cell infiltration

目的研究VI型胶原蛋白α1(COL7A1)在肺腺癌中的表达、预后价值以及和肿瘤微环境中免疫细胞浸润的关系。方法使用TCCA和CEO数据库的RNA-Seq数据和临床信息,分析COL7A1mRNA表达。蛋白质组分析评估COL7A1蛋白表达。受试者工作特征(ROC)曲线用于评估COL7A1在肺腺癌的诊断价值。通过Cox回归和Kaplan-Meier生存分析评估COL7A1的预后价值,并建立了临床预测模型。TIME2.0数据库用于评估COL7A1与免疫细胞浸润的相关性。RT-qPCR验证了COL7A1的表达情况。结果COL7A1在肺腺癌组织中明显上调,特别是在远处转移和晚期的组织中更为明显。COL7A1具有肺腺癌诊断和预后价值,COL7A1高表达是肺腺癌的独立危险因素。构建的预测模型较TNM分期更准确。此外,COL7A1与肿瘤成纤维细胞浸润正相关,与中性粒细胞浸润负相关。结论COL7A1在肺腺癌中高表达提示预后不良,可能成为免疫治疗的潜在靶点。

Objective To investigate the expression of COL7A1 in lung adenocarcinoma,its prognostic value,and its relationship with immune cell infiltration in the tumor microenvironment.Methods RNA-Seq data and clinical information from TCGA and GEO databases were analyzed to assess COL7A1 mRNA expression.Protein expression was evaluated through proteomic analysis.The diagnostic value of COL7A1 in lung adenocarcinoma was assessed using ROC curves.Prognostic value was evaluated using Cox regression and Kaplan-Meier survival analyses,and a clinical prediction model was established.The TIME2.0 database was used to evaluate the correlation between COL7A1 and immune cell infiltration.RT-qPCR was performed to validate COL7A1 expression.Results COL7A1 was significantly upregulated in lung adenocarcinoma tissues,particularly in those with distant metastasis and at advanced stages.COL7A1 showed diagnostic and prognostic value in lung adenocarcinoma,with high COL7A1 expression being an independent risk factor.The constructed prediction model was more accurate than TNM staging.Additionally,COL7A1 was positively correlated with tumor fibroblast infiltration and negatively correlated with neutrophil infiltration.Conclusion High expression of COL7A1 in lung adenocarcinoma indicates poor prognosis and suggests that COL7A1 could be a potential target for immunotherapy.