尿液分析仪在临床检验中影响尿液检验的因素分析

Analysis of Factors Influencing Urine Testing by Urine Analyzer in Clinical Testing

目的:分析在临床检验中应用尿液分析仪实施尿液检验的相关影响因素。方法:随机选取157例2022年7月~2023年6月在本院进行尿液检验的患者,尿液检验工具为全自动尿液分析仪,比较尿液标本不同放置时间以及不同温度下检验结果,同时分析尿液检验结果影响因素。结果:相比于尿液样本即时检查结果,放置30min后检查结果(包括白细胞含量、pH值、尿比重、亚硝酸盐阳性及尿糖阳性检出率)无显著差异(P>0.05),尿液样本放置60min各项指标值均高于即时检查及放置30min,差异有统计学意义(P<0.05)。25℃温度下尿液检验结果显示白细胞阳性率、尿酮体阳性率及尿糖阳性率均高于4℃~8℃,差异有统计学意义(P<0.05)。共计17例患者尿液样本检出误差,误差总发生率达10.83%,其中,实验室因素为主要诱因,尿液采集因素、临床用药因素以及样本保存因素也属于重要诱因,样本送检率最低。结论:尿液分析仪检验结果影响因素较多,为了保证检验结果的准确性必须规范检验流程,妥善保存,及时送检,以降低检验误差发生率。

Objective:To analyze the relevant influencing factors of applying urine analyzers to urine testing in clinical trials.Methods:157 patients who underwent urine tests in our hospital from July 2022 to June 2023 were randomly selected,apply a fully automated urine analyzer to perform urine testing,compare urine test results at different storage times and temperatures,and analyze the influencing factors of urine test results.Results:There was no statistically significant difference in the detection rates of white blood cell content,pH value,urine specific gravity,nitrite positivity,and urine glucose positivity between immediate examination and 30 minutes of storage of urine samples(P>0.05).The white blood cell content,pH value,and urine specific gravity values of urine samples were higher after 60 minutes of storage,and the rates of nitrite positivity and urine glucose positivity were higher than those of immediate examination and 30 minutes of storage,with a statistically significant difference(P<0.05).The urine test results at 25˚C showed that the positive rates of white blood cells,urinary ketone bodies,and urine glucose were all higher than those at 4˚C-8˚C,with statistically significant differences(P<0.05).A total of 17 patients had errors in urine sample detection,with a total error rate of 10.83%.Among them,laboratory factors accounted for the highest proportion,followed by urine collection,clinical medication,and sample preservation.The proportion of samples sent for testing was the lowest.Conclusion:There are many factors that affect the results of urine analyzer testing.In order to ensure the accuracy of the test results,it is necessary to standardize the testing process,properly store them,and send them for testing in a timely manner to reduce the incidence of testing errors.