摘要
对节肢动物鼠妇内生真菌Talaromyces malicola的次生代谢产物进行研究,采用硅胶柱色谱、凝胶柱色谱、半制备液相色谱等进行分离纯化,共从鼠妇内生真菌T.malicola发酵物的乙酸乙酯部位中分离鉴定出11个化合物。结合核磁共振波谱、高分辨质谱、紫外、红外以及圆二色谱等分析方法确定了化合物的结构,分别为talarosesquiterpene A(1)、(3β,5α,6α,15α,22E)-5,6-epoxyergosta-8(14),22-diene-3,7,15-triol(2)、vermistatin(3)、hydroxyvermistatin(4)、bercheminol A(5)、penicillide(6)、lunatinin(7)、penipurdin A(8)、emodin(9)、BE-25327(10)、(-)-regiolone(11)。化合物1为新的diaporol型倍半萜类化合物,化合物2、4~5、7~11为首次从篮状菌属真菌中分离得到。
The secondary metabolites of the endophytic fungus Talaromyces malicola hosted in the arthropod Armadillidium vulgare were separated by silica gel column chromatography,gel column chromatography,and semi-preparative high-performance liquid chromatography.Eleven compounds(1-11)were obtained from the ethyl acetate fraction of the fermentation broth of T.malicola,and their structures were identified by NMR,HR-ESI-MS,UV,IR,and ECD.The 11 compounds were talarosesquiterpene A(1),(3β,5α,6α,15α,22E)-5,6-epoxyergosta-8(14),22-diene-3,7,15-triol(2),vermistatin(3),hydroxyvermistatin(4),bercheminol A(5),penicillide(6),lunatinin(7),penipurdin A(8),emodin(9),BE-25327(10),and(-)-regiolone(11).Compound 1 was a new diaporol-type sesquiterpene.Compounds 2,4-5,and 7-11 were isolated from Talaromyces for the first time.
作者
杨晨宇
陈科良
刘洋
易诚
贾兴宝
刘云宝
YANG Chen-yu;CHEN Ke-liang;LIU Yang;YI Cheng;JIA Xing-bao;LIU Yun-bao(State Key Laboratory of Bioactive Substance and Function of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第16期4470-4476,共7页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(22207127)。