优质蛋白玉米‘荃玉9号’主要营养品质性状的QTL定位

The Quality Protein Maize‘Quanyu No.9’:QTL Mapping of Main Nutritional Quality Traits

油分、蛋白质和淀粉是玉米籽粒的主要营养成分,同时也是玉米重要的品质性状。为探索更多玉米品质相关的QTL,本研究以优质蛋白玉米‘荃玉9号’构建的F2:3群体为材料,通过方差分析和QTL定位方法,对玉米籽粒品质性状(包括油分、蛋白质和淀粉含量)进行了遗传分析。在新都和简阳2地共检测到24个品质性状QTL,其中仅与油分含量、蛋白质含量和淀粉含量相关的QTL分别为9、9和2个,与多个品质性状相关的QTL为4个,QTL在10条染色体上均有分布,单个QTL可解释的表型贡献率为1.92%~34.43%。染色体上Bin7.01(6399330~8305989)的QTL在2个环境同时被检测到,可以解释12.68%~18.13%的表型变异且有加性效应,是控制玉米油分含量的主效QTL。本研究为玉米品质改良的分子辅助育种工作提供了基础数据资料,为后续的育种实践提供了有力支撑。

Oil,protein and starch are the main nutritional components of maize kernels,and they are also important quality traits of maize.In order to explore more QTLs related to maize quality,the F2:3 population constructed by quality protein maize'Quanyu No.9'was used as the material to measure the quality traits in Xindu and Jianyang.QTL analysis was performed using ICIM mapping4.1 software.In this study,a total of 24 QTLs for quality traits were detected in the two locations,of which 9,9 and 2 QTLs were only associated with oil content,protein content and starch content,respectively,and 4 QTLs were associated with multiple quality traits.These QTLs were distributed on 10 chromosomes,and the phenotypic contribution rate explained by a single QTL was 1.92%-34.43%.The QTL located in Bin7.01(6399330-8305989)on the chromosome was detectable in both environments,explaining 12.68%-18.13%of phenotypic variation with additive effects.It is the major QTL controlling oil content in corn.This study provides basic data for molecular assisted breeding of maize quality improvement and provides strong support for subsequent breeding practice.