转录组和代谢组联合分析阐释木薯叶片花青素合成机制

Explanation of Integrative Analysis of Metabolome and Transcriptome for Anthocyanins Synthesis Mechanism in Cassava Leaves

木薯是世界上第六大粮食作物,其块根富含淀粉但缺乏蛋白质、花青素、胡萝卜素等营养物质。为了探索木薯花青素生物合成机制,本研究选取了两种不同颜色木薯种质资源叶片(FL与PL)为材料,进行转录组和花青素靶向代谢组及其联合分析。转录组分析结果显示在FL和PL中6864个差异表达基因,其中包含4112个上调表达和2752个下调表达。代谢组分析结果显示26种显著差异代谢物在PL中显著高于FL,其中21种属于花青素类。联合分析结果显示,其中7个差异表达的基因与花青素生物合成相关,且花青素含量与差异基因的表达呈正相关,尤其是MeANS1的表达差异最大。本研究结果为阐明木薯花青素的生物合成机制提供了候选基因,同时也为提高木薯花青素含量奠定科学基础。

Cassava is the sixth largest staple food crop in the world,which storage root is rich in starch while lack of proteins,anthocyanins and carotenes and so on.Two types of leaves of cassava with different color(FL and PL)were selected as materials to explore the mechanism of anthocyanin biosynthesis by combining with transcriptome and metabolome in this work.The transcriptome results showed that there were 6864 differentially expressed genes(DEGs)including 4112 up-regulating and 2752 down-regulating in PL compare with FL.The metabolome results showed that there were 26 significantly different metabolites(SDMs)in PL compare with FL,and 21 of them belonged to anthocyanins.The result of combination of transcriptome and metabolome showed that there were seven DEGs related to anthocyanin biosynthesis and all of them were positively correlated to anthocyanin biosynthesis.The expression of MeANS1 showed the most difference.It provided candidate genes for elucidating the mechanism of anthocyanin biosynthesis in cassava and laid a scientific foundation for enhancement content of anthocyanin in future.