为害北苍术根螨的种类鉴定与防治初探

Preliminary study on molecular identification and control of root mites on Atractulodes chinensis(DC.)Koidz

为明确河北省深泽县北苍术种植田根螨的种类、为害情况,以及使用苏云金杆菌对其的田间防效,开展了调查与试验,采用5点取样法调查根螨为害情况,利用mtCOⅠ和ITS2分子标记鉴定其种类,施用苏云金杆菌JQD117悬浮剂进行田间防治。结果表明:深泽县北苍术根螨mtCOⅠ和ITS2核苷酸序列与罗宾根螨mtCOⅠ和ITS2核苷酸序列的一致性分别达98%以上和99%以上,成螨形态特征也与罗宾根螨一致,由此确定北苍术根螨为罗宾根螨Rhizoglyphus robini Claparede。罗宾根螨严重为害调查田的北苍术,死苗率达12%。JQD117对北苍术根螨的防效为85.63%,表现出较好的生防应用潜力。

This study aims to clarify the species and damage of root mites on Atractulodes chinensis(DC.)Koidz,as well as the efficacy of Bacillus thuringiensis as a control agent on root mites in Shenze County,Hebei Province,a investigation and experimental trial were conducted.The damage causing by the root mites to A.chinensis was investigated using a five-point sampling method,and the species were identified using mtCO Ⅰ and ITS2 molecular markers.At the same time,the root mites on A.chinensis were controlled in the field with a Bt JQD117 aqueous solution.The results showed that the nucleotide sequences of mtCO Ⅰ and IITS2 of A.chinensis root mite in Shenze Country exhibited a high consistency with those of Rhizoglyphus robini,exceeding 98% and 99%,respectively.The morphological characteristics of the adult mites were also found to be consistent with those of R.robini.Therefore,the A.chinensis root mite was identified as R.robini.The damage rate of R.robini on A.chinensis was 7.0%-26.0%,resulting in a mortality rate of up to 12% for A.chinensis.The control effect of Bt JQD117 aqueous solution on the root mites of A.chinensis was 85.63%,providing a scientific basis for the green control of the root mites of A.chinensis in the future.