郏县红牛线粒体全基因组测序及结构特征分析

Whole genome sequencing and structural characterization of mitochondria of Jiaxian County red cattle

郏县红牛是我国八大优良黄牛品种之一,是当地一个役肉兼用的优良品种。利用高通量测序技术对郏县红牛全线粒体基因组进行测序,并对其特征进行分析。结果显示:郏县红牛全线粒体基因组全长16 339 bp,共注释到37个基因(包含13个蛋白编码基因、22个tRNA、2个rRNA)和一个控制区域(D-loop),无内含子区域,但有重叠基因,A+T含量为60.6%,存在明显的AT偏向性。基因组序列中共存在13个基因间隔区域,最长为16 bp,位于NADH5与NADH6之间;同时存在4个基因重叠,最长为33 bp,位于tRNA-Gln与tRNA-Met之间。其中9个基因位于轻链,26个基因位于重链。郏县红牛线粒体基因组序列的解析有助于丰富其线粒体基因组资源,同时为郏县红牛的种质资源保护和遗传育种提供重要的基础。

Red cattle of Jiaxian County is one of the eight excellent yellow cattle breeds in China,and it is an excellent local breed that can be used for both serving and meat.The whole mitochondrial genome of Jiaxian red cattle was sequenced by high-throughput sequencing technology,and its characteristics were analyzed.The results showed that the whole mitochondrial genome of Jiaxian red cattle was 16 339 bp in length,and 37 genes(including 13 protein coding genes,22 tRNAs,and 2 rRNAs) and a control region(D-loop) were annotated.There were no intron regions,but there were overlapping genes.The A + T content was 60.6%,and there was obvious AT bias.There are 13 gene spacer regions in the genome sequence,the longest of which is 16 bp,located between NADH5 and NADH6.At the same time,there are four overlapping genes,the longest of which is 33 bp,located between tRNA-Gln and tRNA-Met.Nine of these genes are located in the light chain and 26 genes are located in the heavy chain.The analysis of mitochondrial genome sequence of Jiaxian red cattle is helpful to enrich its mitochondrial genome resources,and provides an important foundation for germplasm resources protection and genetic breeding of Jiaxian red cattle.