响应面法优化有机溶剂提取虾青素的工艺研究

Optimization of Astaxanthin extraction with organic solvent by response surface method

为确定有机溶剂提取虾青素的最佳条件,本研究选用破壁后的雨生红球藻,提取选用有机溶剂,以物料比、皂化液浓度、皂化时间、虾青素提取时间为指标,采用单因素和响应面法优化实验条件。结果表明,最佳工艺参数为:提取溶剂为乙酸乙酯-乙醇(1∶1)、料液比为1∶288(g∶mL)、皂化液浓度为0.05mol·L^(-1)、皂化时间为12h,虾青素平均提取率为3.32%。采用响应面法优化有机溶剂提取虾青素的工艺具有可行性,且提取的虾青素纯度较高,杂质少,可用于虾青素的提取。

In order to determine the best condition of extracting Astaxanthin with organic solvent,this study selected Haematococcus pluvialis after breaking the wall,and extracted with organic solvent,the experimental conditions were optimized by single factor and response surface methodology(RSM) with material ratio,saponification solution concentration,saponification time and Astaxanthin extraction time as indexes.The results showed that the optimum technological parameters were:solvent was ethyl acetate-ethanol(1∶1),material-liquid ratio was 1∶288(g∶mL),saponification solution concentration was 0.05mol·L^(-1),saponification time was 12h,and the average extraction rate of Astaxanthin was 3.32%.It is feasible to optimize the extraction process of astaxanthin with organic solvent by response surface methodology,and the extracted Astaxanthin has higher purity and less impurities,which can be used for Astaxanthin extraction.