植物乳杆菌和糖蜜接种对串叶松香草青贮的影响

Effects of Lactiplantibacillus plantarum and molasses on the silage of Silphium perfoliatum

【目的】研究植物乳杆菌与糖蜜接种对串叶松香草青贮发酵特征及绵羊瘤胃降解率的影响,为串叶松香草的科学高效利用提供依据。【方法】设计3个添加剂处理,分别为无添加剂接种处理(CK处理)、接种植物乳杆菌1×10^(5)CFU/g FM(Lactiplantibacillus plantarum,LP处理)、接种植物乳杆菌1×10^(5)CFU/g FM+2%糖蜜(Lactiplantibacillus plantarum and Molasses,LPM处理)。采用真空袋法调制串叶松香草青贮,室温储藏60 d,评价感官质量并分析发酵特性、营养品质及绵羊瘤胃降解率。【结果】(1)LPM处理的乳酸、乙酸、粗蛋白、干物质含量显著高于CK和LP处理(P<0.05)。LPM处理的中性、酸性洗涤纤维、pH值、氨态氮总氮、酵母菌、霉菌、好氧细菌数量显著低于CK和LP处理(P<0.05)。LP和LPM处理的丙酸含量均显著低于CK处理(P<0.05)(2)瘤胃降解过程中(12~48 h),LPM处理的酸性、中性洗涤纤维降解率、干物质降解率均显著高于CK处理(P<0.05),而且酸性、中性洗涤纤维降解率、干物质降解率、有机物降解率在瘤胃中随着时间的增加显著上升(P<0.05)。【结论】联合接种1×10^(5)CFU/g植物乳杆菌和2%糖蜜可促进串叶松香草青贮发酵,并显著提高干物质和纤维在羊瘤胃中的降解率。

【Objective】This project aims to explore the effects of Lactiplantibacillus plantarum and molasses inoculation on silage fermentation characteristics of Silphium perfoliatum and rumen degradation rate of sheep and guide the scientific and efficient utilization of Silphium perfoliatum.【Methods】Three additive treatments were designed:no additive inoculation treatment(CK treatment),inoculation of Lactiplantibacillus plantarum 1×10^(5)CFU/g FM(Lactiplantibacillus plantarum,LP treatment),inoculation of Lactiplantibacillus plantarum 1×10^(5)CFU/g FM+2%molasses(Lactiplantibacillus plantarum and Molasses,LPM treatment).The vacuum bag method was used to prepare the cross-leaf rosin grass silage,which was stored at room temperature for 60 days.Afterwards,the sensory quality was evaluated and the fermentation characteristics,nutritional quality and rumen degradation rate of sheep were analyzed.【Results】(1)The contents of lactic acid,acetic acid,crude protein and dry matter in LPM treatment were significantly higher than those in CK and LP treatments(P<0.05).The neutral detergent fiber,acid detergent fiber,pH value,ammonia nitrogen ratio total nitrogen,yeast,mold and aerobic bacteria in LPM treatment were significantly lower than those in CK and LP treatment(P<0.05).The propionic acid content of LP and LPM treatments was significantly lower than that of CK treatment(P<0.05).(2)During the rumen degradation process(12-48 h),the acid,neutral detergent fiber degradation rate and dry matter degradation rate of LPM treatment were significantly higher than those of CK treatment(P<0.05),and the acid,neutral detergent fiber degradation rate,dry matter degradation rate and organic matter degradation rate increased significantly with time in the rumen(P<0.05).【Conclusion】The combined inoculation of 1×10^(5)CFU/g Lactiplantibacillus plantarum and 2%molasses can promote the fermentation of Silphium perfoliatum silage and significantly improve the degradation of dry matter and fiber in the rumen of sheep.