掌叶覆盆子TIFY基因家族鉴定及表达分析

Identification and Expression Profiles of TIFY Gene Family in Rcbus chingii Hu

为鉴定掌叶覆盆子(Rubus chingii Hu)TIFY基因家族成员,明确它们在不同组织、果实不同发育时期及茉莉酸甲酯(MeJA)处理下的表达水平,本研究通过生物信息学技术确定掌叶覆盆子基因组中的TIFY家族成员,对它们的理化性质、保守基序、基因结构、系统进化树、染色体定位、顺式作用元件等进行了系统分析,并结合转录组和实时荧光定量PCR比较了TIFY家族基因在不同组织、果实发育不同时期以及MeJA诱导后的表达水平。结果表明,掌叶覆盆子基因组中有16个TIFY家族成员,不均匀地分布在7条染色体上,编码118~534个氨基酸,分为4个亚族,主要定位于细胞核中。同一亚族成员基因结构、保守基序和蛋白质二级结构相类似。RcTIFY家族基因的启动子区域存在大量激素响应和胁迫应答相关的顺式作用元件。RcTIFY家族基因在掌叶覆盆子不同组织中均有表达,且具有明显的组织特异性,在果实不同发育时期和MeJA处理下的表达量均存在显著差异,RcJAZ2在果实中特异高表达,并受MeJA强烈诱导,与类黄酮物质在掌叶覆盆子果实中的积累规律相一致,推测可能与果实中类黄酮物质的积累有关。上述结果为后续研究RcTIFY基因家族在类黄酮生物合成中的功能奠定了基础。

To identify the members of TIFY gene family in Rubus chingii Hu and elucidate their expression patterns in different tissues,during vavious growth stages of fruits,and under methyl jasmonate(MeJA)treatment.Bioinformatics were employed to identify the TIFY family members in the R.chingii genome.Their physicochemical properties,conserved motifs,gene structure,phylogenetic tree,chromosomal localization,and cis-acting elements were investigated.Additionally,their expression profiles in different tissues,at different developmental stages,and under treatment with methyl jasmonate(MeJA)were compared using transcriptome analysis and quantitative real-time fluorescence polymerase chain reaction.The results showed that a total of 16 TIFY family members were excavated,which were unevenly distributed on 7 chromosomes,encoding proteins ranging from 118 to 534 amino acids.These proteins were classified into four subfamilies and predominantly targeted to the nucleus.The gene structure,conserved motifs,and secondary structure of RcTIFY members within the same subfamily were similar.Numerous cis-acting elements,including hormone responsiveness and stress responsiveness,were found in the promoter regions of RcTIFY family genes.RcTIFY family genes were differentially expressed in various tissues(roots,stems,leaves,flowers,and fruits),at different fruit development stages(small green fruits,big green fruits,yellow fruits,and red fruits),and under the treatment of MeJA.Notably,RcJAZ2 was highly expressed in fruits of R.chingii,and induced by MeJA,suggesting its involvement in the accumulation of flavonoids.The results laid a foundation for in-depth study on the role of RcTIFY family genes in R.chingii.