魔芋海藻糖-6-磷酸合酶基因家族鉴定及表达分析

Genomic Identification and Expression Analysis of Trehalose-6-Phosphate Synthase Gene Family in Amorphophallus konjac

海藻糖-6-磷酸合成酶(TPS)在调节植物抗逆性及生长发育等方面具有重要作用。为探究TPSs家族在魔芋生长发育及其次生代谢中的作用,本研究分析了魔芋AkTPSs家族成员的蛋白理化性质、系统发育、基因结构、染色体定位及共线性关系,以及它们在魔芋不同组织器官、球茎不同生长发育时期的表达水平,利用实时荧光定量PCR(qRT-PCR)检测筛选出的目标AkTPSs在镧诱导下的表达情况。结果表明,15个魔芋AkTPSs基因随机分布在5条染色体上,其蛋白序列的氨基酸长度为278~867,均为亲水蛋白。系统进化树显示,魔芋AkTPSs被分为2个亚家族,与水稻OsTPSs亲缘关系较近,且存在基因串联复制现象。启动子调控元件分析结果显示,AkTPSs含多个与植物激素、生长发育等相关的顺式元件。结合转录组数据与镧诱导后的qRT-PCR分析,筛选出了与魔芋球茎生长发育及其葡甘聚糖积累密切相关的AkTPS14。本研究结果为魔芋AkTPSs基因及其生物学功能的深入研究提供了理论参考。

Trehalose-6-phosphate Synthase(TPSs)play an important role in plant growth and stress tolerance.To investigate the role of the AkTPSs family in the growth and secondary metabolism of Amorphophallus konjac(A.konjac),the physicochemical properties,phylogenetic relationships,gene structure,chromosomal localization,and protein collinearity of the AkTPSs family were analyzed.Additionally,the expression levels of AkTPSs in various tissues and during corm growth stages of A.konjac were investigated.Quantitative real�time PCR(qRT-PCR)was utilized to assess the expression of selected AkTPSs targets following lanthanum induction.The results revealed that 15 AkTPSs genes were randomly distributed in 5 chromosomes.The proteins encoded by these genes vary in length from 278 to 867 amino acids,with all the AkTPSs being hydrophilic.The AkTPSs proteins were divided into 2 subfamilies with distinct functions by phylogenetic analysis,showing close relationships with OsTPSs proteins.AkTPSs have gene tandem duplications.Promoter cis-acting element analysis indicated that AkTPSs contain several cis-elements related to plant hormones,growth,and other functions.Transcriptome data combined with lanthanum-induced qRT-PCR analysis revealed that AkTPS14 played significant roles in regulating both the growth and the accumulation of glucan in the A.konjac corm.These results provide a theoretical reference for further studies on AkTPSs genes and their biological functions in A.konjac.