骨科学领域组织透明化技术的应用与展望

Applications and prospects of tissue clearing technology in field of orthopedics

背景:骨组织作为高度矿化且结构复杂的结缔组织,在维持生物体形态和功能中扮演关键角色,传统的成像技术难以满足对其深入观察的需求。组织透明化技术的出现使得研究者能够更加清晰地观察到骨内的细微结构,如骨小梁、骨髓腔以及与骨组织相互作用的神经和血管网络,为骨科学的研究和临床应用开辟了新的视角。目的:旨在全面梳理组织透明化技术在骨科学领域的应用,并探讨其在骨组织结构与功能研究、疾病机制解析以及治疗策略创新中的潜力,同时对其创新点进行展望。方法:检索覆盖中国知网和PubMed数据库,英文检索词为“tissue clearing,tissue optical,bone”等,中文检索词为“组织透明化、骨、骨缺损”等,结合布尔逻辑以优化检索策略。纳入与组织透明化技术和骨科学研究直接相关的期刊论文和学位论文,排除关联性不强、重复或数据不完整的文献。最终纳入82篇文献进行分析,关注点包括透明化技术的效能、应用范围及对骨科学研究的贡献。结果与结论:①组织透明化技术在骨结构分析、骨代谢研究、骨肿瘤的病理学特征、骨折愈合过程、骨感染的机制研究以及骨移植和植入物材料的生物相容性评价等多个领域均展现出其独特的应用优势;②该技术的应用不仅加速了骨科学领域的基础研究进展,也为临床治疗提供了新的策略和方法,其在骨科学研究领域具有广阔的应用前景。

BACKGROUND:Bone tissue,as a highly mineralized and structurally complex connective tissue,plays a pivotal role in maintaining the form and function of living organisms.Traditional imaging techniques have been insufficient to meet the demands for in-depth observation of bone.The advent of tissue clearing technology has enabled researchers to more clearly observe the intricate structures within bones,such as trabecular bone,bone marrow cavities,and neural and vascular networks interacting with bone tissue.This has opened new perspectives for research and clinical applications in orthopedics.OBJECTIVE:To comprehensively summarize the applications of tissue clearing technology in the field of orthopedics and to explore its potential in the study of bone tissue structure and function,elucidation of disease mechanisms,and innovation in treatment strategies.Additionally,it provides an outlook on innovative directions.METHODS:The review encompassed a search of the CNKI and PubMed databases using search terms“tissue clearing,tissue optical,bone”in English and“tissue clearing,bone,bone defect”in Chinese,combined with Boolean logic to optimize the search strategy.The inclusion criteria were scholarly articles and dissertations directly related to tissue clearing technology and orthopedic research,excluding literature with weak relevance,duplication,or incomplete data.A total of 82 articles were finally analyzed,focusing on the efficacy of clearing techniques,their scope of application,and their contributions to orthopedic research.RESULTS AND CONCLUSION:(1)Tissue clearing technology has demonstrated unique advantages in various domains,including the analysis of bone structure,the study of bone metabolism,the pathological characteristics of bone tumors,the process of fracture healing,the mechanisms of bone infection,and the evaluation of biocompatibility of bone grafts and implant materials.(2)The application of this technology has not only accelerated the progress of basic research in the field of orthopedics but also provides new strategies and methods for clinical treatment,indicating a broad application prospect in orthopedic research.