硫自养反硝化脱氮技术对含氮污水的处理研究

Research on Sulfur Autotrophic Denitrification Technology for Treating Nitrogen-containing Wastewater

为考察硫自养反硝化脱氮工艺技术对含氮污水的处理效果,本文采用此技术对模拟含氮污水进行脱氮实验处理,其间,分别考察了水力停留时间、进水总氮浓度负荷、反应温度、进水pH等因素对脱氮处理效果的影响,同时对硫自养污泥菌群结构组成进行了微生物群落分析。作为种泥中起硝化作用的Nitrospira(硝化螺菌属),异养反硝化作用的Methylotenera(甲烷氧化菌属)分别由种泥中5.55%和3.46%的占比下降至驯化后的0.63%和0.17%,硝化细菌和异养反硝化细菌基本消失,作为自养反硝化细菌的Thiobacillus(脱氮硫杆菌属)明显富集,硫自养反硝化体系基本驯化完成。结果表明,硫自养反硝化技术,经实验验证考察,适应温差范围大,耐受进水pH幅度较高,覆盖进水总氮浓度浮动大,可有效实现污水总氮去除,减少药耗。

In order to investigate the treatment effect of sulfur autotrophic denitrification technology on nitrogencontaining wastewater,this paper used this technology to conduct denitrification experiments on simulated nitrogencontaining wastewater.Among them the effects of hydraulic retention time,total nitrogen concentration load of influent,reaction temperature,influent pH and other factors on denitrification treatment effect were investigated,and the microbial community structure and composition of sulfur autotrophic sludge were analyzed.As Nitrospira(Nitrospira genus)that plays a role in nitrification in the seed mud,Methanotenera(Methanotenera genus)that undergoes heterotrophic denitrification has decreased from 5.55%and 3.46%in the seed mud to 0.63%and 0.17%after domestication.Nitrifying bacteria and heterotrophic denitrification bacteria have basically disappeared.Thiobacillus(Thiobacillus genus),as an autotrophic denitrifying bacterium,is significantly enriched,and the sulfur autotrophic denitrification system has been basically domesticated.The results show that the sulfur autotrophic denitrification technology process,after experimental verification and investigation,does not require the addition of organic carbon sources,adapts to a large temperature difference range,tolerates a high pH range in the influent,and has a large fluctuation in the total nitrogen concentration in the covered influent.It can effectively achieve the removal of total nitrogen in wastewater and Reduce drug consumption.