红嘴鸥和喜鹊的粪便微生物群多样性比较研究

Comparative Study on the Fecal Microbiota Diversity of Black-Headed Gull and Magpie

为评估不同种鸟类的病原体传播风险,提取红嘴鸥和喜鹊粪便的总DNA,通过PCR扩增16S rRNA序列V3~V4高变区,基于高通量测序和生物信息学研究两者肠道微生物群差异。研究结果显示,红嘴鸥和喜鹊粪便微生物群组成存在显著差异,两者粪便中最丰富的细菌门分别是厚壁菌门和变形菌门,前者粪便中绝对优势属是Catellicoccus(丰度均值65.5%)。红嘴鸥样本中均检测出弯曲杆菌属,检出率100%,丰度均值1.2%;喜鹊样本检出率55.6%,丰度均值0.019%。这表明,青岛市红嘴鸥种群广泛存在弯曲杆菌属,红嘴鸥的接触安全风险高于喜鹊。

Total DNA from fecal samples were extracted and the V3~V4 hypervariable region of the 16S rRNA sequence was amplified to assess the potential risk of bird-to-human pathogen transmission in different bird species.Gut microbiota characterizations were determined with high-throughput sequencing and the followed bioinformatical analyses.The results show remarkable differences in the fecal microbiota between black-headed gulls and magpies.Firmicutes and Proteobacteria are the primary bacterial phyla in black-headed gull and magpie feces,respectively.Catellicoccus is the predominant genus in black-headed gull feces,and the average abundance is 65.5%.The detective rate of Campylobacter in black-headed gull feces is 100%,and the average abundance is 1.2%.But in magpie feces,the detective rate is 55.6%,and the average abundance is 0.019%.This suggests that Campylobacter is widespread in the population of black-headed gull in Qingdao,and the safety risk of exposure to black-headed gulls is higher than magpies.