5个环状RNAs表达水平与甲状腺乳头状癌临床特征的关联分析

Correlation analysis between expression levels of five circRNAs and clinical features of papillary thyroid carcinoma

目的:分析甲状腺乳头状癌(PTC)组织中5个目标环状RNAs的表达水平及其与临床特征的关系,探索其在PTC和良性结节(BN)鉴别诊断中的价值。方法:收集来源于同一患者的PTC和BN组织标本共30组,采用实时荧光定量PCR检测目标环状RNAs表达水平并进行差异分析;将30例PTC患者根据不同的临床病理特征进行分组,比较目标环状RNAs组间表达差异;分析目标环状RNAs表达量与术前临床指标水平之间的相关性;受试者工作特征(ROC)曲线分析目标环状RNAs对PTC和BN的鉴别诊断效能。结果:PTC组织中hsa_circ_0008838、hsa_circ_0030716、hsa_circ_0131483、hsa_circ_0053378和chr1:39927555|39934404的相对表达量均高于BN组织(P<0.05)。有BRAFV600E突变的PTC组织中hsa_circ_0030716表达水平低于没有BRAFV600E突变的组织,pN1b期PTC组织中hsa_circ_0131483表达水平高于pN0和pN1a期,多灶性PTC组织中chr1:39927555|39934404表达水平高于单灶性,以上差异均有统计学意义(P<0.05)。hsa_circ_0053378和TSH为正相关;hsa_circ_0053378和T3为负相关;hsa_circ_0131483和T4为负相关,差异均有统计学意义(P<0.05)。Hsa_circ_0030716,hsa_circ_0008838和hsa_circ_0131483鉴别诊断PTC和BN的AUC值分别为0.929,0.867和0.895。结论:PTC组织中目标环状RNAs表达增加,并与临床特征相关;hsa_circ_0030716,hsa_circ_0008838和hsa_circ_0131483对PTC和BN有较高的鉴别诊断价值。

Objective:To analyse the expression levels of five target circRNAs(hsa_circ_0008838,hsa_circ_0030716,hsa_circ_0131483,hsa_circ_0053378,and chr1:39927555|39934404)in papillary thyroid carcinoma(PTC)tissues and their correlation with clinical characteristics and to explore its value in the differential diagnosis of PTC and benign nodules(BN).Methods:A total of 30 groups of PTC and BN tissues from the same surgical patient were collected.The expression levels of target circular RNAs were detected by real-time fluorescence quantitative PCR and the differences were analyzed.Group the 30 PTC patients based on different clinicopathological characteristics,compare the differences in expression levels of target circRNAs between groups,and calculate the correlation betw een the expression levels of target circRNAs and preoperative clinical indicators.Receiver Operating Characteristic(ROC)curve was used to analyze the differential diagnostic efficacy of target circRNAs for PTC and BN.Results:In PTC tissues,the expression levels of hsa_circ_0008838,hsa_circ_0030716,hsa_circ_0131483,hsa_circ_0053378 and chr1:39927555|39934404 were higher than those in BN tissues(P<0.05).In PTC tissues with the BRAFV600E mutation,the expression level of hsa_circ_0030716 was lower compared to tissues without the BRAFV600E mutation.In pN1b stage PTC tissues,the expression level of hsa_circ_0131483 was higher than in pN0 and pN1a stages.In multifocal PTC tissues,the expression level of chr1:39927555|39934404 was higher than in solitary PTC tissues.All these differences were significant with P<0.05.Hsa_circ_0053378 showed a positive correlation with TSH,a negative correlation with T3,and hsa_circ_0131483 showed a negative correlation with T4.All these correlations were significant with P<0.05.The AUC values for discriminating between PTC and BN for hsa_circ_0030716,hsa_circ_0008838 and hsa_circ_0131483 were 0.929,0.867 and 0.895,respectively.Conclu sion:The expression of target circRNAs is increased in PTC tissues and correlated with clinical features.hsa_circ_0030716,hsa_circ_0008838 and hsa_circ_0131483 have high differential diagnostic value for PTC and BN.