芥菜SRO基因家族全基因组鉴定与表达分析

Genome-wide Identification and Expression Analysis of the SRO Gene Family in Brassica juncea L.

【目的】SRO基因家族是植物特有的一类转录因子,在植物生长发育与胁迫响应中具有重要作用,研究芥菜SRO基因家族,为解析芥菜SRO基因功能与遗传改良提供理论依据。【方法】利用生物信息学鉴定油用芥菜与菜用芥菜基因组中的SRO基因家族成员,运用TBtools、MEGA、Cytoscape、NCBI、STRING、EggNOG等软件与数据库进行理化性质、序列特征、进化关系、调控网络等分析以及RT-qPCR分析盐胁迫下的表达模式。【结果】两种类型芥菜SRO基因家族成员数量存在差异,并分属A与B两大类,其中A类SRO蛋白含有WWE、PARP及RST结构域,而B类蛋白则缺少WWE结构域,SRO基因启动子区域含有多种非生物胁迫响应与激素响应的顺式作用元件,microRNA-BjuSRO-靶基因构建复杂调控网络,参与了细胞凋亡、根系形态建成、免疫应答、异源刺激的细胞反应、对活性氧的反应等生物学过程,油用芥菜BjuOSRO基因与甘蓝SRO基因具有较近的进化关系,RT-qPCR结果显示,在盐胁迫下BjuVA1a、BjuVA1e、BjuVA2a、BjuVA3a、BjuVB1b、BjuVA2a显著上调表达。【结论】芥菜SRO基因家族具有功能多样性,BjuVA1a、BjuVA1e、BjuVA2a、BjuVA3a、BjuVB1b、BjuVA2a与盐胁迫响应密切相关,可作为培育耐盐型芥菜新品种的候选基因。

【Objective】SRO gene family is a class of plant-specific transcription factors,which plays an important role in plant growth and development and stress response.The study of the SRO gene family in Brassica juncea L.provides a theoretical basis for analyzing the function of SRO genes in B.juncea L.and its genetic improvement.【Method】Bioinformatics was used to identify members of the SRO gene family in the genomes of two types of oilseed mustard and vegetable mustard,the software and database such as TBtools,MEGA,Cytoscape,NCBI,STRING,EggNOG were used for the analysis of physicochemical properties,sequence characterization,evolutionary relationships,regulatory networks,etc.,as well as RT-qPCR to analyze the expression patterns under salt stress.【Result】There were differences in the number of members of the SRO gene family between the two types of B.juncea L.,and they belonged to two major classes.Among them,class A SRO proteins contained WWE,PARP and RST structural domains,whereas class B proteins were lack of WWE domains.The promoter region of the SRO genes contained a variety of cis-acting elements for abiotic stress response and hormone response.The microRNA-BjuSRO-target genes constructed a complex regulatory network,which was involved in apoptosis,root morphogenesis,immune response,cellular response to heterologous stimuli,and response to reactive oxygen species,and other biological processes.The RT-qPCR results showed that BjuVA1a,BjuVA1e,BjuVA2a,BjuVA3a,BjuVB1b,and BjuVA2a were significantly upregulated under salt stress.【Conclusion】The SRO gene family in the mustard has functional diversity,and BjuVA1a,BjuVA1e,BjuVA2a,BjuVA3a,BjuVB1b,and BjuVA2a are closely related to the response to salt stress,which can be used as candidate genes for breeding new salt-tolerant varieties of B.juncea L.