NPCl-CDs/Fe^(3+)荧光探针的制备及对L-Cys的传感检测

Preparation of NPCl-CDs/Fe^(3+)Fluorescent Probe and the Detection for L-Cys

以葡萄糖、乙二胺、浓盐酸和浓磷酸作为反应前体,采用一步水热法合成了一种蓝绿色的荧光碳点NPCl-CDs,并以此构建了NPCl-CDs/Fe^(3+)荧光探针用于实际样品中L-半胱氨酸(L-Cys)的定量检测.实验结果表明,在NPCl-CDs中加入Fe^(3+)可使NPCl-CDs的荧光猝灭,当向NPCl-CDs/Fe^(3+)猝灭体系中引入L-Cys时,体系的荧光强度得以恢复.由此构建了一种用于L-Cys定量检测的新型“开-关-开”NPCl-CDs/Fe^(3+)-L-Cys荧光传感系统.该方法在5.8~60.0μmol/L浓度范围内呈现较宽的线性区域,检出限为0.052μmol/L.该荧光传感系统对L-Cys表现出良好的选择性,对实际样品中L-Cys的检测具有潜在的应用价值.

In this work,a blue-green NPCl-CDs was synthesized by one-step hydrothermal method using glucose,ethylenediamine,concentrated hydrochloric acid and concentrated phosphoric acid as reaction precursors.Then NPCl-CDs/Fe^(3+)fluorescent probe was constructed for the quantitative detection of L-Cys in actual samples.The fluorescence intensity of NPCl-CDs could be quenched after addition of Fe^(3+).The fluorescence intensity of the NPCl-CDs/Fe^(3+)system was restored when L-Cys was introduced into the system.Therefore,a novel"on-off-on"NPCl-CDs/Fe^(3+)-L-Cys fluorescence sensing system for L-Cys quantitative detection was constructed.This experiment showed a wide linear region in the concentration range of 5.8—60.0μmol/L with a detection limit of 0.052μmol/L.The results indicated that the fluorescence sensing system had good selectivity,sensitivity and potential application value for the detection of L-Cys in real samples.