三七对下腔静脉结扎DVT大鼠模型D-二聚体、cTn-I、BNP的影响

Effects of Radix Notoginseng on D-dimer,cTn-I and BNP in rat model of DVT with inferior vena cava ligation

目的观察三七对下腔静脉结扎深静脉血栓(DVT)大鼠D-二聚体、心肌肌钙蛋白I(cTn-I)、血B型钠尿肽(BNP)的影响。方法将20只大鼠随机分为三七组、DVT组、依诺肝素组和假手术组,每组各5只。除假手术组外均行开腹结扎下腔静脉造模。下腔静脉结扎造模成功后48 h处死大鼠,取游离左肾静脉和下腔静脉汇合处及向下1 cm血管进行苏木素-伊红染色,于显微镜下观察,并检测血管D-二聚体、cTn-I、BNP含量。结果四组大鼠血浆cTn-I、D-二聚体、BNP含量比较,差异均有统计学意义(F分别=26.56、9.86、7.34,P均<0.05)。DVT组大鼠血浆中cTn-I、D-二聚体、BNP含量高于假手术组,差异有统计学意义(t分别=11.40、6.67、8.92,P均<0.05)。三七组、依诺肝素组cTn-I含量明显低于DVT组,差异均有统计学意义(t分别=2.62、3.50,P均<0.05)。依诺肝素组D-二聚体含量明显低于DVT组,差异有统计学意义(t=3.66,P<0.05)。结论三七和依诺肝素对于DVT大鼠模型均有一定治疗作用。

Objective To observe the effects of Radix Notoginseng on D-dimer,cTn-I and BNP in DVT rats.Methods Twenty rats were randomly divided into four groups,namely,the Radix Notoginseng group,the DVT group,the enoxaparin group and the sham group,with five animals in each group,and all of them were subjected to open-abdomen ligation of the inferior vena cava for modeling except the sham-operation group,which was only subjected to open-abdomen.The animals were executed 48h after the ligation and modeling of inferior vena cava,and the blood vessels at the confluence of the free left renal vein and inferior vena cava and 1 cm downward were stained with hematoxylin-eosin,and observed under the microscope.The plasma D-dimer,cTn-I,and BNP were detected.Results Comparison of plasma cTn I,D-dimer,and BNP levels among the four groups of rats showed statistically significant differences(F=26.56,9.86,7.34,P<0.05).The cTn-I,D-dimer,and BNP levels of rats in the DVT group were higher than those in the sham group,and the differences were statistically significant(t=11.40,6.67,8.92,P<0.05).Compared with the DVT group,the cTn-I content in the Radix Notoginseng group and enoxaparin group was significantly lower(t=2.62,3.50,P<0.05).The D-dimer content in the enoxaparin group was significantly lower than that in the DVT group,and the difference was statistically significant(t=3.66,P<0.05).Conclusion Both Radix Notoginseng and enoxaparin had some therapeutic effects on the DVT rat model.