1,8-二氨基萘衍生化GC-MS法检测血液中亚硝酸盐及其稳定性

Determination of blood nitrite and its stability by 1,8-Diaminonaphthalene derivatized GC-MS method

目的建立亚硝酸盐中毒死亡案例血液的气相色谱-质谱法(GC-MS)并考察在血液中亚硝酸盐的稳定性,为推断死亡时血液中亚硝酸盐浓度及判定是否为亚硝酸盐中毒死亡提供参考依据。方法采用1,8-二氨基萘(1,8-DAN)衍生化GC-MS法检测亚硝酸盐中毒死亡的血液检材;取空白血液添加亚硝酸盐标准,定期测定亚硝酸盐含量,观察血液中亚硝酸盐的稳定性,得出其浓度变化趋势。结果11例亚硝酸盐中毒死亡案例血液中亚硝酸盐浓度为1.165~351.551μg/mL;随时间增加,加标血样中亚硝酸盐浓度逐渐降低。结论本研究所建立检测方法操作简便、准确度高、精密度好;亚硝酸盐在血液中不稳定,建议尽快检测,以防漏检。

Objective To establish a gas chromatography-mass spectrometry(GC-MS)method for blood samples of death cases with nitrite poisoning and examine the stability of nitrite in blood,so as to provid a reference for inferring the concentration of nitrite in blood at the time of death and determining whether the death was caused by nitrite poisoning.Methods The 1,8-diaminonaphthalene(1,8-DAN)derivatized GC-MS method was used to detect the blood samples of people who died of nitrite poisoning;Blank blood was taken with nitrite standards,and the content was measured regularly.The stability of nitrite in blood was observed,and its concentration change trend was obtained.Results The concentration in blood of 11 cases of nitrite poisoning deaths ranged from 1.165μg/mL to 351.551μg/mL;With the increase of time,the concentration of nitrite in the labeled blood samples gradually decreased.Conclusion The detection method established in this research is easy to operate,has high accuracy and good precision.Nitrite is unstable in blood,it is recommended to detect it as soon as possible to prevent missed detection.